Fucoidin inhibits attachment of guinea pig spermatozoa to the zona pellucida through binding to the inner acrosomal membrane and equatorial domains.
Fucoidin inhibits attachment of guinea pig spermatozoa to the zona pellucida through binding to the inner acrosomal membrane and equatorial domains.
复制标题
岩藻多糖通过与内顶体膜和赤道区结合，抑制豚鼠精子附着在透明带上。
DOI：
10.1016/0014-4827(84)90606-2
复制
发表时间：
1984
影响因子：
3.7
通讯作者：
Yanagimachi,R
中科院分区：
文献类型：
--
作者：
Huang,TT;Yanagimachi,R
我们以前的研究表明，岩藻多糖，藻类杂多糖，是一种有效的抑制精子结合在豚鼠，仓鼠和人类。为了使岩藻多糖结合的表面位点可视化，制备天然岩藻多糖的生物素化衍生物（B-Fuc）。B-Fuc保留了抑制活性，并与FITC-亲和素结合使用荧光显微镜定位其在豚鼠精子上的结合位点。在活的顶体反应的精子中，B-Fuc主要结合于顶体内膜和赤道段结构域。该结合被10倍过量的天然岩藻多糖有效地竞争，但不被10倍过量的肝素或20倍过量的生物素化正常兔血清IgG竞争。B-Fuc在死精子上的结合模式与活精子的结合模式有很大不同。顶体后区，而不是顶体内膜和赤道域，强烈标记。这表明尽可能使用活细胞评估真实表面结合位点的重要性。我们的结论是，内顶体膜和/或赤道域是至关重要的，在豚鼠中的结合。
Our previous study has shown that fucoidin, an algal heteropolysaccharide, is a potent inhibitor of sperm-zona binding in the guinea pig, hamster and human. To visualize the surface site of fucoidin binding, a biotinated derivative (B-Fuc) of the native fucoidin was prepared. B-Fuc retained the inhibitory activity and was used in conjunction with FITC-avidin to localize its binding sites on guinea pig spermatozoa using fluorescence microscopy. In living acrosome-reacted spermatozoa, B-Fuc bound predominantly to the inner acrosomal membrane and equatorial segment domains. The binding was effectively competed by a 10-fold excess of native fucoidin, but not by a 10-fold excess of heparin or a 20-fold excess of biotinated normal rabbit serum IgG. B-Fuc binding patterns on dead spermatozoa were quite different from that of living spermatozoa. The post-acrosomal region, rather than the inner acrosomal membrane and equatorial domains, was intensely labeled. This indicates the importance of using living cells in assessing true surface binding sites whenever possible. We conclude that the inner acrosomal membrane and/or equatorial domains are critical for zona binding in the guinea pig.