Nucleotide sequence, organisation and structural analysis of the products of genes in the nirB-cysG region of the Escherichia coli K-12 chromosome.
Nucleotide sequence, organisation and structural analysis of the products of genes in the nirB-cysG region of the Escherichia coli K-12 chromosome.
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大肠杆菌 K-12 染色体 nirB-cysG 区域基因产物的核苷酸序列、组织和结构分析。
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发表时间：
1990
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通讯作者：
Jeffrey A. Cole
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作者：
Timothy Peakman;Joel Crouzet;J. Mayaux;Stephen J. W. Busby;S. Mohan;Nerina R. Harborne;John C. Wootton;Rachel E. Nicolson;Jeffrey A. Cole
大肠杆菌染色体的74分钟区域中的5618个碱基区域的DNA序列和衍生的氨基酸序列已经确定，以定位NADH依赖性亚硝酸盐还原酶的结构基因nirB和合成sirohaem辅基所需的基因cysG。在nirB和cysG之间发现了三个额外的开放阅读框架nirD、nirE和nirC。通过比较推导的氨基酸序列与数据库蛋白的氨基酸序列，推导了NirB蛋白的NADH和FAD的潜在结合位点，以及保守的中央核心和界面结构域。一个直接重复的序列，其中包括基序-Cys-Xaa-Xaa-Cys-，被认为是一个[4Fe-4S]或两个[2Fe-2S]簇的结合位点。nirD基因可能编码一种功能未知的可溶性细胞质蛋白。没有显着的相似性之间的衍生氨基酸序列的NirD和任一NirB或任何其他蛋白质的数据库。如果nirE开放阅读框被翻译，它将编码一个33个氨基酸的未知功能的肽，其中包括8个苯丙氨酰残基。nirC基因的产物是一种高度疏水的蛋白质，其氨基酸序列区域与细胞色素氧化酶多肽1相似。
The DNA sequence and derived amino-acid sequence of a 5618-base region in the 74-min area of the Escherichia coli chromosome has been determined in order to locate the structural gene, nirB, for the NADH-dependent nitrite reductase and a gene, cysG, required for the synthesis of the sirohaem prosthetic group. Three additional open reading frames, nirD, nirE and nirC, were found between nirB and cysG. Potential binding sites on the NirB protein for NADH and FAD, as well as conserved central core and interface domains, were deduced by comparing the derived amino-acid sequence with those of database proteins. A directly repeated sequence, which includes the motif -Cys-Xaa-Xaa-Cys-, is suggested as the binding site for either one [4Fe-4S] or two [2Fe-2S] clusters. The nirD gene potentially encodes a soluble, cytoplasmic protein of unknown function. No significant similarities were found between the derived amino-acid sequence of NirD and either NirB or any other protein in the database. If the nirE open reading frame is translated, it would encode a 33-amino-acid peptide of unknown function which includes 8 phenylalanyl residues. The product of the nirC gene is a highly hydrophobic protein with regions of amino-acid sequence similar to cytochrome oxidase polypeptide 1.