Vascular-specific increase in exon 1B-encoded CAV1.2 channels in spontaneously hypertensive rats.
Vascular-specific increase in exon 1B-encoded CAV1.2 channels in spontaneously hypertensive rats.
复制标题
自发性高血压大鼠中外显子 1B 编码的 CAV1.2 通道的血管特异性增加。
DOI：
10.1016/j.amjhyper.2006.01.020
复制
发表时间：
2006
影响因子：
3.2
通讯作者：
Palade,Philip
中科院分区：
文献类型：
--
作者：
Wang,Wen-Ze;Saada,Nehad;Dai,Bosong;Pang,Li;Palade,Philip
背景已知成年自发性高血压大鼠 (SHR) 的脉管系统比正常血压 Wistar京都 (WKY) 大鼠的脉管系统表达更多功能性 L 型 Ca 通道，但尚不清楚哪种 CaV1.2 通道亚型上调。方法采用蛋白质印迹和实时逆转录酶 - 聚合酶链反应 (RT-PCR) 比较成年 SHR 和成年 SHR 选定组织中 CaV1.2 通道蛋白和信息的表达水平。 WKY 大鼠。 结果结果表明 SHR 血管系统中特别是短外显子 1b 编码的氨基末端 CaV1.2 亚型过度表达。表达相同亚型的大脑和内脏平滑肌没有受到类似的影响。信息水平的差异不足以解释异构体特异性蛋白质水平的差异。结论我们得出结论，SHR 脉管系统必须在转录后调节通道。需要进一步的实验来确定这是否涉及更有效地从外显子 1b 特异性转录本翻译蛋白质，更有效地翻译后陪伴到表面膜，或者比 WKY 脉管系统更慢地选择性降解蛋白质的短氨基末端形式。
BackgroundThe vasculature of the adult spontaneously hypertensive rat (SHR) is known to express more functional L-type Ca channels than the vasculature of normotensive Wistar Kyoto (WKY) rats, but it is not known which CaV1.2 channel isoform is upregulated.MethodsWestern blots and real-time reverse transcriptase–polymerase chain reaction (RT-PCR) were used to compare the expression levels of CaV1.2 channel protein and message in selected tissues of adult SHR and WKY rats.ResultsThe results indicate overexpression in SHR vasculature specifically of the short exon 1b-encoded amino terminus CaV1.2 isoform. Brain and visceral smooth muscle expressing the same isoform were not similarly affected. Differences in message levels are insufficient to account for the differences in isoform-specific protein levels.ConclusionsWe conclude that SHR vasculature must regulate the channel postranscriptionally. Further experiments will be required to determine whether this involves translation of protein from exon 1b-specific transcripts more efficiently, posttranslational chaperoning to the surface membrane more efficiently, or selective degradation of the short amino terminus form of the protein more slowly than in WKY vasculature.