The dynamin-related GTPase Dnm1 regulates mitochondrial fission in yeast
The dynamin-related GTPase Dnm1 regulates mitochondrial fission in yeast
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DOI：
10.1038/13014
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发表时间：
1999-09-01
影响因子：
21.3
通讯作者：
Shaw, JM
中科院分区：
文献类型：
--
作者：
Bleazard, W;McCaffery, JM;Shaw, JM
动力蛋白相关的GTdom Dnm1控制酵母中的线粒体形态。在这里，我们表明，dnm1突变转换成一个平面的“网”的相互连接的小管的线粒体室。我们提出，这种净形态的线粒体分裂缺陷的结果。免疫金标记定位Dnm 1的细胞质面收缩的线粒体小管，似乎是分裂和线粒体小管，似乎最近完成分裂的结束。Dnm1的活性是上位的Fzo1，在线粒体外膜，调节线粒体融合的GTdR。DNM 1突变阻止FZO 1突变株的线粒体断裂。这些发现表明，Dnm 1调节线粒体分裂，组装在线粒体小管的细胞质表面的分裂将发生的网站。
The dynamin-related GTPase Dnm1 controls mitochondrial morphology in yeast. Here we show that dnm1 mutations convert the mitochondrial compartment into a planar 'net' of interconnected tubules. We propose that this net morphology results from a defect in mitochondrial fission. Immunogold labelling localizes Dnm1 to the cytoplasmic face of constricted mitochondrial tubules that appear to be dividing and to the ends of mitochondrial tubules that appear to have recently completed division. The activity of Dnm1 is epistatic to that of Fzo1, a GTPase in the outer mitochondrial membrane that regulates mitochondrial fusion. dnm1 mutations prevent mitochondrial fragmentation in fzo1 mutant strains. These findings indicate that Dnm1 regulates mitochondrial fission, assembling on the cytoplasmic face of mitochondrial tubules at sites at which division will occur.