Sortase enzymes in Gram-positive bacteria.
Sortase enzymes in Gram-positive bacteria.
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DOI：
10.1111/j.1365-2958.2011.07887.x
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发表时间：
2011-12
影响因子：
3.6
通讯作者：
Clubb RT
中科院分区：
文献类型：
--
作者：
Spirig T;Weiner EM;Clubb RT
在革兰氏阳性细菌中，蛋白质使用分选酶展示在细胞表面上。这些半胱氨酸转肽酶将带有适当分选信号的蛋白质连接到细胞表面上战略性定位的氨基。分选酶单独或与其他酶协同工作，将蛋白质附着到细胞壁的跨桥肽上，或将蛋白质连接在一起形成皮利。由于表面蛋白在微生物生理学中起着重要作用，并且经常是毒力因子，因此自从十多年前发现分选酶以来，人们一直在深入研究它们。基于它们的一级序列和功能，分选酶可以被分成不同的家族，称为A类至F类酶。大多数细菌使用一种以上类型的分选酶来精心制作它们的表面，这些分选酶通过识别它们的蛋白质底物中的独特分选信号而非冗余地发挥作用。在这里，我们回顾什么是已知的这些酶的功能和催化的分子基础。特别强调的是“菌毛蛋白”特异性C类分选酶，构建结构复杂的皮利。令人兴奋的新数据表明，这些酶可以使用的底物极其混杂，并且其作用机制具有惊人的多样性。我们还回顾了最近的数据表明，分选酶是针对细胞表面上的特定位点，在那里它们与其他分选酶和辅助因子一起正常工作。
In Gram-positive bacteria proteins are displayed on the cell surface using sortase enzymes. These cysteine transpeptidases join proteins bearing an appropriate sorting signal to strategically positioned amino groups on the cell surface. Working alone, or in concert with other enzymes, sortases either attach proteins to the cross-bridge peptide of the cell wall or they link proteins together to form pili. Because surface proteins play a fundamental role in microbial physiology and are frequently virulence factors, sortase enzymes have been intensely studied since their discovery a little more than a decade ago. Based on their primary sequences and functions sortases can be partitioned into distinct families called class A to F enzymes. Most bacteria elaborate their surfaces using more than one type of sortase that function non-redundantly by recognizing unique sorting signals within their protein substrates. Here we review what is known about the functions of these enzymes and the molecular basis of catalysis. Particular emphasis is placed on ‘pilin’ specific class C sortases that construct structurally complex pili. Exciting new data have revealed that these enzymes are amazingly promiscuous in the substrates that they can employ and that there is a startling degree of diversity in their mechanism of action. We also review recent data that suggest that sortases are targeted to specific sites on the cell surface where they work with other sortases and accessory factors to properly function.
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影响因子：
4.8
作者：
Budzik, Jonathan M.;Oh, So-Young;Schneewind, Olaf
通讯作者：
Schneewind, Olaf
影响因子：
3.6
作者：
Capstick, David S.;Willey, Joanne M.;Elliot, Marie A.
通讯作者：
Elliot, Marie A.
影响因子：
4.8
作者：
Budzik, Jonathan M.;Oh, So-Young;Schneewind, Olaf
通讯作者：
Schneewind, Olaf
影响因子：
3.2
作者：
Bagnoli, Fabio;Moschioni, Monica;Barocchi, Michele A.
通讯作者：
Barocchi, Michele A.
影响因子：
11.4
作者：
DeDent, Andrea;Bae, Taeok;Schneewind, Olaf
通讯作者：
Schneewind, Olaf