Atto-Molar Raman detection on patterned superhydrophilic-superhydrophobic platform via localizable evaporation enrichment
Atto-Molar Raman detection on patterned superhydrophilic-superhydrophobic platform via localizable evaporation enrichment
复制标题
通过局部蒸发富集在图案化超亲水-超疏水平台上进行阿托摩尔拉曼检测
DOI：
10.1016/j.snb.2020.128826
复制
发表时间：
2021-01-01
影响因子：
8.4
通讯作者：
Zhong, Minlin
中科院分区：
文献类型：
--
作者：
Luo, Xiao;Pan, Rui;Zhong, Minlin
超低浓度溶液中的分子检测在污染物监测、爆炸物检测、疾病早期诊断等诸多领域都具有重要意义。近年来，研究表明，表面增强拉曼散射（SERS）与超疏水表面结合，能够突破扩散极限，实现在飞托/阿托-摩尔范围内的检测。然而，复杂和昂贵的制造方法限制了大规模的实际应用。在这方面，利用超快激光制备的图案化超亲-超疏水平台可以简化制备过程并固定液滴，引起了人们的关注。然而，报道的相关工作无法实现阿托-摩尔检测。因此，需要具有简化制造工艺、按需定位分析物和超高灵敏度（1am）的SERS平台。在本文中，我们通过飞秒激光处理了一个新的图案SERS平台，实现了阿托摩尔检测，增强因子为1.09 x 10（14）。蒸发富集后，靶分子沉积在约0.01 mm(2)的超亲水性区域上。通过比较Ag纳米粒子、Au纳米粒子和Au纳米星三种胶体，进一步优化SERS底物，发现Au纳米星表现最好。在10(-14)mol/L条件下，相对标准偏差（RSD）为11.7%。该结果为生物传感器超低浓度分子检测提供了有价值的信息和潜力。
The detection of molecules in ultra-low concentration solution is essential in numerous fields including pollutant monitoring, explosive detection and disease early diagnosis. Recently, studies indicated that surface-enhanced Raman scattering (SERS) combining with superhydmphobic surfaces were capable of breaking diffusion limit, realizing detection in femto/atto-molar range. However, the complicated and costly fabrication methods limited larger-scale practical application. In this regard, patterned superhydrophilic-superhydrophobic platform fabricated by ultrafast laser, which could simplify the preparation process and fix the droplet, attracted attention. Nevertheless, the related works reported cannot realize atto-molar detection. Therefore, the SERS platform with abilities of simplified fabrication processes, on-demand locating analyte and ultra-high sensitivity (1 aM) is needed. In this paper, we processed a new patterned SERS platform via femtosecond laser, achieving atto-molar detection with an enhancement factor of 1.09 x 10(14). After evaporation enrichment, the target molecules were deposited on the superhydrophilic area of similar to 0.01 mm(2). We further optimized the SERS substrates by comparing three types of colloids: Ag nano-particles, Au nano-particles and Au nano-stars, finding Au nano-stars exhibited the best. Meanwhile, this platform showed good uniformity: the relative standard deviation (RSD) was 11.7 % at 10(-14)mol/L. The results offer valuable information and potentials on the ultra-low concentration molecular detection in biosensors.