Induction of Chromosomal Translocations with CRISPR-Cas9 and Other Nucleases: Understanding the Repair Mechanisms That Give Rise to Translocations.
Induction of Chromosomal Translocations with CRISPR-Cas9 and Other Nucleases: Understanding the Repair Mechanisms That Give Rise to Translocations.
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DOI：
10.1007/978-981-13-0593-1_2
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发表时间：
2018
影响因子：
--
通讯作者：
Jasin M
中科院分区：
文献类型：
--
作者：
Brunet E;Jasin M
染色体易位与几种肿瘤类型相关，包括造血系统恶性肿瘤、肉瘤和上皮来源的实体瘤，这是由于它们激活原癌基因或产生具有致癌潜力的新型融合蛋白。在许多情况下，由于难以重现融合蛋白的精确表达或其他原因，一直缺乏合适的人类模型的可用性。此外，了解易位如何形成机制一直是一个目标，因为它可能会提出预防其发生的方法。当两条异源染色体上的双链断裂（DSB）的DNA末端不正确连接时，就会发生染色体易位。本文综述了DSB修复机制及其对易位形成的贡献，已用于产生易位的各种可编程核酸酶平台，以及在这一领域取得的成功。
Chromosomal translocations are associated with several tumor types, including hematopoietic malignancies, sarcomas, and solid tumors of epithelial origin, due to their activation of a proto-oncogene or generation of a novel fusion protein with oncogenic potential. In many cases, the availability of suitable human models has been lacking because of the difficulty in recapitulating precise expression of the fusion protein or other reasons. Further, understanding how translocations form mechanistically has been a goal, as it may suggest ways to prevent their occurrence. Chromosomal translocations arise when DNA ends from double-strand breaks (DSBs) on two heterologous chromosomes are improperly joined. This review provides a summary of DSB repair mechanisms and their contribution to translocation formation, the various programmable nuclease platforms that have been used to generate translocations, and the successes that have been achieved in this area.
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影响因子：
16.6
作者：
Day TA;Layer JV;Cleary JP;Guha S;Stevenson KE;Tivey T;Kim S;Schinzel AC;Izzo F;Doench J;Root DE;Hahn WC;Price BD;Weinstock DM
通讯作者：
Weinstock DM
DOI：
10.1083/jcb.201401146
发表时间：
2014-09-29
期刊：
The Journal of cell biology
影响因子：
--
作者：
Barton O;Naumann SC;Diemer-Biehs R;Künzel J;Steinlage M;Conrad S;Makharashvili N;Wang J;Feng L;Lopez BS;Paull TT;Chen J;Jeggo PA;Löbrich M
通讯作者：
Löbrich M
影响因子：
56.9
作者：
Jinek, Martin;Chylinski, Krzysztof;Charpentier, Emmanuelle
通讯作者：
Charpentier, Emmanuelle
影响因子：
4.5
作者：
Lagutina, Irina V.;Valentine, Virginia;Grosveld, Gerard C.
通讯作者：
Grosveld, Gerard C.
影响因子：
5.3
作者：
Elliott, B;Richardson, C;Jasin, M
通讯作者：
Jasin, M