Growth factor levels in leukocyte-poor platelet-rich plasma and correlations with donor age, gender, and platelets in the Japanese population
Growth factor levels in leukocyte-poor platelet-rich plasma and correlations with donor age, gender, and platelets in the Japanese population
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DOI：
10.1186/s40634-019-0175-7
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发表时间：
2019-02-02
影响因子：
1.8
通讯作者：
Yamazaki, Masashi
中科院分区：
文献类型：
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作者：
Taniguchi, Yu;Yoshioka, Tomokazu;Yamazaki, Masashi
背景富血小板血浆（PRP）的临床应用已被加速，以研究各种肌肉骨骼疾病的早期恢复。它涉及通过生理浓度的多种生长因子的作用促进组织损伤修复。PRP的组成因许多因素而异，其中可能包括年龄和性别。因此，我们分析了日本受试者PRP中年龄、性别和血小板计数与生长因子之间的相关性。方法抽取39名20 ~ 49岁健康志愿者的外周血，离心制备（每份样品体积为6 mL），年龄（平均±标准差）为33 ± 8.7岁，性别（男：女）为19：20，体重指数（BMI）为22 ± 4.0。用CaCl 2活化后，储存上清液。PRP中的平均血小板计数为41.4 +/- 12.2 x 10（4）/mu L。PRP浓缩率（即，PRP/外周血小板计数）为1.8 ± 0.4倍。生长因子水平采用酶联免疫吸附试验（ELISA）测定血小板源性生长因子-BB、转化生长因子-β 1、血管内皮生长因子、表皮生长因子、成纤维细胞生长因子、胰岛素样生长因子-1和肝细胞生长因子，并通过计算斯皮尔曼秩相关系数（r）对与年龄、性别和PRP血小板计数的相关性进行统计学分析。结果年龄与血小板源性生长因子-BB和胰岛素样生长因子-1呈负相关（r =-0.32，-0.39），性别对生长因子无影响。PRP中的血小板计数与血小板衍生生长因子-BB、转化生长因子-β 1、表皮生长因子和肝细胞生长因子呈正相关（r分别为0.39、0.75、0.71和0.48）。结论血小板聚集性血小板减少症患者的PRP存在个体差异，且受年龄和血小板计数的影响。我们的数据表明，患者的特征占PRP生理活性的差异。
Background Clinical application of platelet-rich-plasma (PRP) has been accelerated to investigate early recovery from various musculoskeletal conditions. It involves the promotion of tissue damage repair through the action of multiple growth factors at physiological concentrations. The composition of PRP differs based on many factors, which may include age and gender. Therefore, we analyzed correlations between age, gender, and platelet counts in PRP with growth factors in Japanese subjects. Method Peripheral blood was drawn from 39 healthy volunteers between 20 and 49 years of age (age, mean +/- standard deviation = 33 +/- 8.7 years; gender ratio, male:female = 19:20; BMI, mean +/- standard deviation = 22 +/- 4.0) and prepared through centrifugation (volume, 6 mL per sample). After being activated with CaCl2, the supernatant was stored. The mean platelet count in PRP was 41.4 +/- 12.2 x 10(4)/mu L. PRP concentration rate (i.e., PRP/peripheral platelet counts) was 1.8 +/- 0.4 times. Growth factor levels (platelet-derived growth factor-BB, transforming growth factor-beta 1, vascular endothelial growth factor, epidermal growth factor, fibroblast growth factor, insulin-like growth factor-1, and hepatocyte growth factor) were measured using enzyme-linked immunosorbent assay (ELISA), and correlations with age, gender, and PRP platelet counts were statistically analyzed by calculating Spearman's rank correlation coefficients (r). Results Age was negatively correlated with platelet-derived growth factor-BB and insulin-like growth factor-1 (r = - 0.32, - 0.39), and gender had no influence on growth factors. Platelet counts in PRP positively correlated with platelet-derived growth factor-BB, transforming growth factor-beta 1, epidermal growth factor, and hepatocyte growth factor (r = 0.39, 0.75, 0.71, and 0.48, respectively). Conclusions This clinical study shows a significant variation of PRP among individual patients and that this variation is influenced by the age and the platelet counts of the subjects. Our data demonstrate that patient characteristics account for the differences in PRP physiological activity.