Copper deficiency induces the upregulation of the copper chaperone for Cu/Zn superoxide dismutase in weanling male rats
Copper deficiency induces the upregulation of the copper chaperone for Cu/Zn superoxide dismutase in weanling male rats
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DOI：
10.1093/jn/133.1.28
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发表时间：
2003-01-01
影响因子：
4.2
通讯作者：
L'Abbé, MR
中科院分区：
文献类型：
--
作者：
Bertinato, J;Iskandar, M;L'Abbé, MR
确定人体缺铜最常用的指标是血清/血浆铜浓度降低以及铜蓝蛋白和铜/锌超氧化物歧化酶（SOD1）活性降低。然而，这些指标受到许多与铜状态无关的因素的影响，并且缺乏检测边缘缺乏所需的敏感性，限制了它们在许多情况下的有用性。在体内，铜插入 SOD1 取决于 SOD1 的铜伴侣 (CCS)。在本研究中，我们探讨了 CCS 的表达水平可能反映铜状态的可能性，从而作为铜营养的有用标记。断奶雄性 Wistar 大鼠喂食正常（5.3 mg Cu/kg 饮食）、中度缺乏（0.84 mg Cu/kg 饮食）或缺乏（0.34 mg Cu/kg 饮食）铜饮食 6 周。喂食适度和缺乏饮食的大鼠在多项血液学测量中显示出差异（P < 0.05），表明这些组中存在不同程度的铜缺乏。缺铜大鼠的肝脏和红细胞 SOD1 活性和体重降低（P < 0.05）。蛋白质印迹分析显示，缺铜大鼠的肝脏和红细胞中 CCS 表达呈剂量依赖性增加（P < 0.05）。我们报告 CCS 蛋白水平作为评估铜状态的新标志物。
The most commonly used indices for determining copper deficiency in humans are reduced serum/plasma copper concentration and decreased activity of ceruloplasmin and Cu/Zn superoxide dismutase (SOD1). However, these indicators are influenced by many factors unrelated to copper status and lack the sensitivity required to detect marginal deficiency, limiting their usefulness in many situations. In vivo, the insertion of copper into SOD1 is dependent on the copper chaperone for SOD1 (CCS). In this study, we explored the possibility that the expression level of CCS may reflect copper status and thus serve as a useful marker of copper nutriture. Weanling male Wistar rats were fed either a normal (5.3 mg Cu/kg diet), moderately deficient (0.84 mg Cu/kg diet) or deficient (0.34 mg Cu/kg diet) copper diet for 6 wk. Rats fed moderate and deficient diets showed differences (P < 0.05) in several hematological measurements, indicating varying degrees of copper deficiency in these groups. Copper-deficient rats had reduced (P < 0.05) liver and erythrocyte SOD1 activity and body weight. Western blot analysis revealed a dose-dependent increase (P < 0.05) in CCS expression in liver and erythrocytes of copper-deficient rats. We report CCS protein level as a novel marker for assessing copper status.