Temporal profile of stem cell division, migration, and differentiation from subventricular zone to olfactory bulb after transient forebrain ischemia in gerbils
Temporal profile of stem cell division, migration, and differentiation from subventricular zone to olfactory bulb after transient forebrain ischemia in gerbils
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DOI：
10.1097/01.wcb.0000050060.57184.e7
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发表时间：
2003-03-01
影响因子：
6.3
通讯作者：
Abe, K
中科院分区：
文献类型：
--
作者：
Iwai, M;Sato, K;Abe, K
神经发生的阶段可分为三个步骤：增殖、迁移和分化。为了阐明缺血后这三个步骤的详细关系，在成年沙土鼠脑短暂前脑缺血5分钟后，在脑室下区（SVZ）通过吻侧迁移流（RMS）到嗅球（013）的三个步骤进行了综合评价。溴脱氧尿苷（BrdU），高聚唾液酸化神经细胞粘附分子（PSA-NCAM），神经元核抗原（NeuN），胶质细胞酸性蛋白（GFAP）分别作为增殖，迁移和分化的标志物。短暂缺血后，SVZ区BrdU标记的PSA-NCAM阳性细胞数和PSA-NCAM阳性细胞集落面积均增加，10 d达高峰。在RMS中，当RMS体积增大，周围GFAP阳性细胞增多时，共表达PSA-NCAM的BrdU标记细胞数量增加，并在30 d后达到高峰。013在30和60 d时出现BrdU + NeuN双阳性细胞。NeuN染色和末端脱氧核苷酸dUTP缺口末端标记染色显示短暂缺血后SVZ周围、RMS和013中无神经元细胞丢失。这些结果表明，短暂前脑缺血增强SVZ中的神经干细胞增殖，而没有明显的神经元细胞损失，并具有潜在的神经元前体迁移与激活GFAP阳性细胞通过RMS到OB。
The stage of neurogenesis can be divided into three steps: proliferation, migration, and differentiation. To elucidate their detailed relations after ischemia, the three steps were comprehensively evaluated, in the subventricular zone (SVZ) through the rostral migratory stream (RMS) to the olfactory bulb (013), in adult gerbil brain after 5 minutes of transient forebrain ischemia. Bromodeoxyuridine (BrdU), highly polysialylated neural cell adhesion molecule (PSA-NCAM), neuronal nuclear antigen (NeuN), and glial fibrillary acidic protein (GFAP) were used as markers for proliferation, migration, and differentiation, respectively. The number of BrdU-Iabeled cells that coexpressed PSA-NCAM and the size of PSA-NCAM-positive cell colony increased in the SVZ with a peak at 10 d after transient ischemia. In the RMS, the number of BrdU-Iabeled cells that coexpressed PSA-NCAM increased, with a delayed peak at 30 d, when the size of RMS itself became larger and the number of surrounding GFAP-positive cells increased. In the 013, BrdU + NeuN double positive cells were detected at 30 and 60 d. NeuN staining and terminal deoxynucleotidyl dUTP nick-end labeling staining showed no neuronal cell loss around the SVZ, and in the RMS and the 013 after transient ischemia. These findings indicate that transient forebrain ischemia enhances neural stem cell proliferation in the SVZ without evident neuronal cell loss, and has potential neuronal precursor migration with activation of GFAP-positive cells through the RMS to the OB.