Neurons derived from human mesenchymal stem cells show synaptic transmission and can be induced to produce the neurotransmitter substance P by interleukin-1α
Neurons derived from human mesenchymal stem cells show synaptic transmission and can be induced to produce the neurotransmitter substance P by interleukin-1α
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DOI：
10.1634/stemcells.2004-0251
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发表时间：
2005-03-01
期刊：
影响因子：
5.2
通讯作者：
Rameshwar, P
中科院分区：
文献类型：
--
作者：
Cho, KJ;Trzaska, KA;Rameshwar, P
间充质干细胞（MSC）表现出免疫抑制特性，遵循多谱系分化模式，并表现出转分化潜力。间充质干细胞易于从成人骨髓中扩增，并且与伦理问题无关，这使得间充质干细胞在临床应用中具有吸引力。根据突触素免疫染色和电生理学研究，用视黄酸处理的间充质干细胞导致突触传递。原位杂交表明神经递质基因前原速激肽-I在这些细胞中表达。然而，该基因的翻译仅在白细胞介素 (IL)-1 α 刺激后发生。 IL-1 受体拮抗剂的共刺激减弱了这种作用。这项研究报告了间充质干细胞转分化为具有功能性突触的神经元的能力，并有可能极化产生特定的神经递质。
Mesenchymal stem cells (MSCs) exhibit immune-suppressive properties, follow a pattern of multilineage differentiation, and exhibit transdifferentiation potential. Ease in expansion from adult bone marrow, as well as its separation from ethical issues, makes MSCs appealing for clinical application. MSCs treated with retinoic acid resulted in synaptic transmission, based on immunostaining of synaptophysin and electrophysiological studies. In situ hybridization indicated that the neurotransmitter gene preprotachykinin-I was expressed in these cells. However, translation of this gene only occurred after stimulation with interleukin (IL)-1 alpha. This effect was blunted by costimulation with IL-1 receptor antagonist. This study reports on the ability of MSCs to be transdifferentiated into neurons with functional synapses with the potential to become polarized towards producing specific neurotransmitters.