Molecular ties between the cell cycle and differentiation in embryonic stem cells
Molecular ties between the cell cycle and differentiation in embryonic stem cells
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DOI：
10.1073/pnas.1408638111
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发表时间：
2014-07-01
影响因子：
11.1
通讯作者：
Kirschner, Marc W.
中科院分区：
文献类型：
--
作者：
Li, Victor C.;Kirschner, Marc W.
在体细胞分化的最后阶段，达到分化状态与细胞周期进程密切相关。关于细胞周期在胚胎发育早期阶段的作用知之甚少。在这里，我们表明，涉及细胞周期的分子途径可以被工程化，以强烈影响胚胎干细胞分化的早期阶段在体外。基于干扰这些途径的策略可以缩短ES分化的速率并简化ES分化的谱系路径。这些结果使得涉及细胞增殖的途径与调控胚胎细胞谱系的途径在不同点上交叉，并证明这种知识可以有益地用于指导多能细胞的细胞分化的途径和有效性。
Attainment of the differentiated state during the final stages of somatic cell differentiation is closely tied to cell cycle progression. Much less is known about the role of the cell cycle at very early stages of embryonic development. Here, we show that molecular pathways involving the cell cycle can be engineered to strongly affect embryonic stem cell differentiation at early stages in vitro. Strategies based on perturbing these pathways can shorten the rate and simplify the lineage path of ES differentiation. These results make it likely that pathways involving cell proliferation intersect at various points with pathways that regulate cell lineages in embryos and demonstrate that this knowledge can be used profitably to guide the path and effectiveness of cell differentiation of pluripotent cells.