INTEGRATION OF HEPATITIS-B VIRUS-DNA - EVIDENCE FOR INTEGRATION IN THE SINGLE-STRANDED GAP
INTEGRATION OF HEPATITIS-B VIRUS-DNA - EVIDENCE FOR INTEGRATION IN THE SINGLE-STRANDED GAP
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DOI：
10.1016/0092-8674(83)90152-6
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发表时间：
1983-01-01
期刊：
影响因子：
64.5
通讯作者：
HOFSCHNEIDER, PH
中科院分区：
文献类型：
--
作者：
KOSHY, R;KOCH, S;HOFSCHNEIDER, PH
B型肝炎病毒（HBV）DNA可整合到感染的人肝细胞基因组中，并可能与原发性肝癌（PLC）的发生发展有关。本文报道了从人肝PLC细胞系PLC/PRF/5的DNA中克隆的3个HBV DNA整合序列的分析。在所有这些序列中，病毒DNA的整合是在未整合的HBV DNA中的单链（缺口）位点。与一些推测相反，在逆转录病毒前病毒中发现的插入片段两侧没有大的末端冗余。病毒序列在这些克隆中没有显示出明显的重排，但整合可能与病毒DNA中的缺失有关，也明显与宿主DNA中的缺失有关。
The DNA of hepatitis B virus (HBV) can integrate into the genomes of infected human liver cells and may be related to the development of primary liver carcinoma (PLC). This report describes the analysis of 3 integrated HBV DNA sequences cloned from DNA of the human liver PLC cell line PLC/PRF/5. In all these sequences, integration of the viral DNA is at a site that is single-stranded (gap) in the unintegrated HBV DNA. Contrary to some speculations, there are no large terminal redundancies flanking the inserts as found in retroviral proviruses. The viral sequences show no obvious rearrangements in these clones, but integration can be associated with deletions in the viral DNA and also apparently in the host DNA.