Potent in vitro activity of the albumin fusion type 1 interferons (albumin-interferon-alpha and albumin-interferon-beta) against RNA viral agents of bioterrorism and the severe acute respiratory syndrome (SARS) virus.
Potent in vitro activity of the albumin fusion type 1 interferons (albumin-interferon-alpha and albumin-interferon-beta) against RNA viral agents of bioterrorism and the severe acute respiratory syndrome (SARS) virus.
复制标题
白蛋白融合 1 型干扰素（白蛋白-干扰素-α 和白蛋白-干扰素-β）具有对抗生物恐怖分子 RNA 病毒和严重急性呼吸综合征 (SARS) 病毒的有效体外活性。
DOI：
10.1159/000140361
复制
发表时间：
2008
期刊：
影响因子：
3.3
通讯作者：
Sidwell,RobertW
中科院分区：
文献类型：
--
作者：
Subramanian,GMani;Moore,PaulA;Gowen,BrianB;Olsen,AaronL;Barnard,DaleL;Paragas,Jason;Hogan,RobertJ;Sidwell,RobertW
Background:The type 1 interferons (INF-α and INF-β) are potent antiviral agents. Albumin-INF-α and albumin-INF-β are novel recombinant proteins consisting of IFN-α or IFN-β genetically fused to human albumin.Methods:The in vitro antiviral activity of albumin-IFN-α was evaluated against representative bioterrorism viral agents and the severe acute respiratory syndrome virus. Antiviral activity was assessed using inhibition of cytopathic effect and neutral red staining.Results:EC50values for albumin-IFN-α ranged from <0.1 ng/ml for Punta Toro virus to 65 ng/ml for Venezuelan equine encephalitis virus in the neutral red assay. Albumin-IFN-β showed 75- and 360-fold greater in vitro activity than albumin-IFN-α against Ebola virus and severe acute respiratory syndrome, respectively.Conclusion:Further evaluation of these long-acting albumin-IFN fusion proteins as prophylactic or therapeutic agents against these viral agents of bioterrorism in relevant primate models is warranted.
