UNIVERSAL CHEMICAL-ASSAY FOR THE DETECTION AND DETERMINATION OF SIDEROPHORES
UNIVERSAL CHEMICAL-ASSAY FOR THE DETECTION AND DETERMINATION OF SIDEROPHORES
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DOI：
10.1016/0003-2697(87)90612-9
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发表时间：
1987-01-01
影响因子：
2.9
通讯作者：
NEILANDS, JB
中科院分区：
文献类型：
--
作者：
SCHWYN, B;NEILANDS, JB
利用铁载体对铁（III）的高亲和力，建立了一种检测和测定铁载体的通用方法。在630 nm处，铬azrol S/铁(III)/十六烷基三甲基溴化铵三元配合物的消光系数约为100,000 M-1 cm-1，可作为指示剂。当强螯合剂将铁从染料中去除时，染料的颜色就从蓝色变成橙色。由于灵敏度高，可以直接在培养液的上清液上进行溶液中铁载体的测定和纸电泳色谱的表征。该方法也适用于琼脂平板。蓝色琼脂上菌落周围的橙色光晕表明铁载体排泄。研究表明，大肠杆菌菌株在肠杆菌系统中的生物合成、转运和调节突变是明显可区分的。该方法成功地筛选了两株meliloti根瘤菌DM5和1021的铁吸收系统突变体。
A universal method to detect and determine siderophores was developed by using their high affinity for iron(III). The ternary complex chrome azurol S/iron(III)/hexadecyltrimethylammonium bromide, with an extinction coefficient of approximately 100,000 M-1 cm-1 at 630 nm, serves as an indicator. When a strong chelator removes the iron from the dye, its color turns from blue to orange. Because of the high sensitivity, determination of siderophores in solution and their characterization by paper electrophoresis chromatography can be performed directly on supernatants of culture fluids. The method is also applicable to agar plates. Orange halos around the colonies on blue agar are indicative of siderophore excretion. It was demonstrated with Escherichia coli strains that biosynthetic, transport, and regulatory mutations in the enterobactin system are clearly distinguishable. The method was successfully used to screen mutants in the iron uptake system of two Rhizobium meliloti strains, DM5 and 1021.