The origin of the Haitian cholera outbreak strain.
The origin of the Haitian cholera outbreak strain.
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DOI：
10.1056/nejmoa1012928
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发表时间：
2011-01-06
期刊：
影响因子：
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通讯作者：
Waldor MK
中科院分区：
文献类型：
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作者：
Chin CS;Sorenson J;Harris JB;Robins WP;Charles RC;Jean-Charles RR;Bullard J;Webster DR;Kasarskis A;Peluso P;Paxinos EE;Yamaichi Y;Calderwood SB;Mekalanos JJ;Schadt EE;Waldor MK
虽然霍乱自1991年以来就在拉丁美洲出现，但它至少100年来没有在海地流行。然而，最近海地爆发了严重的霍乱。我们使用第三代单分子实时DNA测序来确定来自海地当前疫情的2种临床霍乱弧菌分离株、1991年在拉丁美洲引起霍乱的1种菌株以及2002年和2008年在南亚分离的2种菌株的基因组序列。使用一级序列数据，我们比较了这5种菌株的基因组和一组先前获得的23种不同霍乱弧菌菌株的部分基因组序列，以评估海地霍乱疫情的可能起源。单核苷酸变异以及高变染色体元件的存在和结构表明，海地分离株与2002年和2008年在孟加拉国分离的变异霍乱弧菌El Tor O1菌株之间存在密切关系。与此相反，海地分离株的基因组变异分析揭示了一个更遥远的关系与循环南美分离株。海地疫情可能是通过人类活动从遥远的地理来源引入霍乱弧菌菌株的结果。（由国家过敏和传染病研究所和霍华德休斯医学研究所资助。）
Although cholera has been present in Latin America since 1991, it had not been epidemic in Haiti for at least 100 years. Recently, however, there has been a severe outbreak of cholera in Haiti. We used third-generation single-molecule real-time DNA sequencing to determine the genome sequences of 2 clinical Vibrio cholerae isolates from the current outbreak in Haiti, 1 strain that caused cholera in Latin America in 1991, and 2 strains isolated in South Asia in 2002 and 2008. Using primary sequence data, we compared the genomes of these 5 strains and a set of previously obtained partial genomic sequences of 23 diverse strains of V. cholerae to assess the likely origin of the cholera outbreak in Haiti. Both single-nucleotide variations and the presence and structure of hypervariable chromosomal elements indicate that there is a close relationship between the Haitian isolates and variant V. cholerae El Tor O1 strains isolated in Bangladesh in 2002 and 2008. In contrast, analysis of genomic variation of the Haitian isolates reveals a more distant relationship with circulating South American isolates. The Haitian epidemic is probably the result of the introduction, through human activity, of a V. cholerae strain from a distant geographic source. (Funded by the National Institute of Allergy and Infectious Diseases and the Howard Hughes Medical Institute.)