Cyclo-oxygenase-2 expression in esophageal adenocarcinoma as a determinant of clinical outcome following esophagectomy.
Cyclo-oxygenase-2 expression in esophageal adenocarcinoma as a determinant of clinical outcome following esophagectomy.
复制标题
食管腺癌中环加氧酶 2 的表达是食管切除术后临床结果的决定因素。
作者：
M. France;P. Drew;T. Dodd;D. Watson
本研究的目的是确定环氧化酶-2 （COX-2）在食管腺癌中的表达是否影响食管切除术患者的生存结果。从阿德莱德大学外科部门维护的食管切除术数据库中确定了10例食管腺癌切除术后存活超过3年的患者。另外一组10名因腺癌接受食管切除术，但在手术后12个月内因疾病复发死亡的患者也被确定。将所有患者的原始福尔马林固定癌组织切片包埋于石蜡块中，并对肿瘤标本切片进行COX-2蛋白免疫组化染色。然后由一名“盲法”病理学家对染色程度进行分级：0级，无染色；1 +级，有限染色；2 +级，强染色。然后构建Kaplan-Meier生存曲线，并用于确定生存与COX-2染色、肿瘤t分期、局部淋巴结转移和肿瘤血管侵袭之间的相关性。95%（19/20）的患者COX-2染色阳性。2 +级染色患者的生存结果明显低于1 +级患者（P = 0.03）。t期恶化、淋巴结转移和血管侵犯也有缩短生存期的趋势。COX-2蛋白似乎在大多数食管腺癌中表达。在这项研究中，COX-2表达水平的升高与较差的生存结果相关。与传统的组织病理学分期相比，COX-2蛋白表达相关性可能是一个更好的食管腺癌预后指标。
The aim of this study was to determine if cyclo-oxygenase-2 (COX-2) expression in adenocarcinoma of the esophagus affects survival outcome in patients undergoing esophagectomy. Ten patients surviving more than 3 years following an esophagectomy for adenocarcinoma of the esophagus were identified from an esophagectomy database maintained by the University of Adelaide Department of Surgery. An additional group of 10 patients, who underwent esophagectomy for adenocarcinoma, but who died within 12 months of surgery due to recurrent disease, were also identified. Pieces of the original formalin fixed carcinoma tissue embedded in paraffin blocks from all of these patients were obtained, and slices of the tumor specimens underwent immunohistochemical staining with COX-2 protein. The extent of staining was then graded by a single 'blinded' pathologist: grade 0, no staining; grade 1 +, limited staining; and grade 2 +, strong staining. Kaplan-Meier survival curves were then constructed and used to determine correlations between survival and COX-2 staining, tumor T-stage, local lymph node metastases, and tumor vascular invasion. Ninety-five percent (19/20) of patients stained positively for COX-2. Patients with grade 2 + staining had a significantly poorer survival outcome compared to grade 1 + patients (P = 0.03). There were also trends towards shorter survival with worsening T-stage, lymph node metastasis and vascular invasion. COX-2 protein appears to be expressed by most esophageal adenocarcinomas. An increased level of expression of COX-2 was associated with a poorer survival outcome in this study. COX-2 protein expression association could be a better prognostic indicator for esophageal adenocarcinoma than traditional histopathological staging.
DOI：
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发表时间：
2006
期刊：
--
影响因子：
--
作者：
K. Wilson;Sidong;Fu;Kalathur;S. Ramanujam;Stephen;J. Meltzer
通讯作者：
K. Wilson;Sidong;Fu;Kalathur;S. Ramanujam;Stephen;J. Meltzer
影响因子：
11.2
作者：
B. Reddy;Y. Hirose;R. Lubet;V. Steele;G. Kelloff;Susan Paulson;Karen Seibert;C. Rao
通讯作者：
B. Reddy;Y. Hirose;R. Lubet;V. Steele;G. Kelloff;Susan Paulson;Karen Seibert;C. Rao
影响因子：
29.4
作者：
Buttar, NS;Wang, KK;Burgart, LJ
通讯作者：
Burgart, LJ