circZMYM2 Competed Endogenously with miR-335-5p to Regulate JMJD2C in Pancreatic Cancer (Retracted article. See vol. 56, pg. 612, 2022)
circZMYM2 Competed Endogenously with miR-335-5p to Regulate JMJD2C in Pancreatic Cancer (Retracted article. See vol. 56, pg. 612, 2022)
复制标题
circZMYM2 与 miR-335-5p 内源性竞争来调节胰腺癌中的 JMJD2C。
DOI：
10.1159/000495868
复制
发表时间：
2018-01-01
影响因子：
--
通讯作者：
He, Xiaozhou
中科院分区：
文献类型：
--
作者：
An, Yong;Cai, Huihua;He, Xiaozhou
背景/目的：研究CircZMYM2(HSA_CIRC_0099999)在胰腺癌(PC)细胞增殖、凋亡和侵袭中的作用，探讨CircZMYM2调控miR-335-5p和JMJD2C的可能机制。方法：用Arraystar Human CircRNA微阵列V1分析20例PC组织和配对正常组织中CircRNA的差异表达。QRT-PCR检测CircZMYM2的表达水平。采用CCK-8比色法、流式细胞仪和Transwell比色法研究了CircZMYM2抑制和过表达对细胞增殖、细胞凋亡和细胞侵袭的影响。用裸鼠进行动物实验，以检测CircZMYM2基因敲除对肿瘤生长的影响。通过RNA下拉实验、双荧光素酶报告基因检测和抢救实验，验证了ZMYM2、miR-335和JMJD2C之间的关系。免疫印迹法检测CircZMYM2和miR-335-5p对JMJD2C蛋白表达的影响。结果：CircZMYM2在PC组织和细胞中均有高表达。CircZMYM2基因敲除后可抑制癌细胞增殖，诱导细胞凋亡，降低癌细胞的侵袭能力。体内肿瘤生长受到抑制。CircZMYM2抑制其靶基因miR-335-5p的表达。MIR-335-5P通过抑制JMJD2C抑制胰腺癌的发展。结论：我们的研究表明，大约ZMYM2促进了PC的进展。CircZMYM2对miR-335-5P有海绵作用，并调控下游癌基因JMJD2C。(C)2018年作者(S)由S.Karger AG，巴塞尔出版
Background/Aims: We aimed to study the involvement of circZMYM2 (hsa_circ_0099999) in pancreatic cancer (PC) cell proliferation, apoptosis and invasion and to figured out the underlying mechanism of circZMYM2 regulating miR-335-5p and JMJD2C. Methods: CircRNA differential expressions in twenty PC samples and paired normal tissue samples were analyzed using Arraystar Human CircRNA microarray V1. CircZMYM2 expression level was determined via qRT-PCR. The effects of circZMYM2 inhibition and overexpression on cell proliferation, cell apoptosis and cell invasion were investigated by CCK-8 assays, Flow cytometry assays and Transwell assays. An animal experiment on nude mice was put forward to test the influence of circZMYM2 knockdown on tumor growth. The relationship between circZMYM2, miR-335 and JMJD2C was verified by RNA pull down, dual-luciferase reporter assays and rescue experiment. The effect of circZMYM2 and miR-335-5p on the expression of JMJD2C protein was detected by western blot. Results: CircZMYM2 overexpression was observed in both PC tissues and cells. Knockdown of circZMYM2 inhibited proliferation, induced apoptosis, and weakened invasion ability of cancer cells. Tumor growth was restrained in vivo. CircZMYM2 repressed the expression of its target miR-335-5p. MiR-335-5p attenuated pancreatic cancer development via inhibition of JMJD2C. Conclusion: Our study demonstrated that circZMYM2 promoted PC progression. CircZMYM2 had a sponge effect on miR-335-5p and modulated the downstream oncogene JMJD2C. (C) 2018 The Author(s) Published by S. Karger AG, Basel