Efficient expression of acetylcholine-binding protein from Aplysia californica in Bac-to-Bac system.
Efficient expression of acetylcholine-binding protein from Aplysia californica in Bac-to-Bac system.
复制标题
加州海兔乙酰胆碱结合蛋白在 Bac-to-Bac 系统中的高效表达
DOI：
10.1155/2014/691480
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发表时间：
2014
影响因子：
--
通讯作者：
Luo S
中科院分区：
文献类型：
--
作者：
Lin B;Meng H;Bing H;Zhangsun D;Luo S
The Bac-to-Bac baculovirus expression system can efficiently produce recombinant proteins, but the system may have to be optimized to achieve high-level expression for different candidate proteins. We reported here the efficient expression of acetylcholine-binding proteins from sea hares Aplysia californica (Ac-AChBP) and a convenient method to monitor protein expression level in this expression system. Three key factors affecting expression of Ac-AChBP were optimized for maximizing the yield, which included the cell density, volume of the infecting baculovirus inoculums, and the culturing time of postinfection. We have found it to reach a high yield of ∼5 mg/L, which needs 55 h incubation after infection at the cell density of 2 × 106 cells/mL with an inoculum volume ratio of 1 : 100. The optimized expression system in this study was also applied for expressing another protein Ls-AChBP from Lymnaea stagnalis successfully. Therefore, this established method is helpful to produce high yields of AChBP proteins for X-ray crystallographic structural and functional studies.
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影响因子：
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作者：
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通讯作者：
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