The Antipruritic Effect of Acupuncture on Serotonin-Evoked Itch in Rats
The Antipruritic Effect of Acupuncture on Serotonin-Evoked Itch in Rats
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DOI：
10.3727/036012908803861168
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发表时间：
2008-01-01
影响因子：
0.3
通讯作者：
Min, Byung-Il
中科院分区：
文献类型：
--
作者：
Han, Jae-Bok;Kim, Chan Woo;Min, Byung-Il
采用大鼠后肢搔抓模型，观察针刺的抗炎作用。针刺或电针（EA），这是进行了30分钟后，痒相关的行为诱导皮内注射2%的血清素（20 μ l）到喙回，然后数的抓挠发作计数为60分钟。在第一个实验中，针刺刺激施加到几个不同的点。然而，针灸显着减少抓挠的数量，只有当应用于颈部皮节。第二个实验是在第一个实验中针刺产生最大抗炎作用的穴位LI 11和1.14处进行普通针刺或2 Hz或120 Hz电针，并以与第一个实验相同的方式给予5-羟色胺。结果表明，2 Hz电针刺激倾向于增加约18%，而120 Hz电针刺激倾向于减少约39%，与动物相比，平原针灸治疗的癫痫发作。用κ-阿片受体拮抗剂去甲binaltorphimine预处理120 Hz电针的镇痛作用，以探讨κ-阿片受体与120 Hz电针镇痛作用的关系，发现去甲binaltorphimine可明显抑制120 Hz电针的镇痛作用。这些结果表明，针灸和EA刺激是有效的治疗瘙痒症，如果给药到相应的受影响的网站或相邻的dermatomes，这种效果是由于最大限度地诱导高频率EA刺激的κ-阿片受体激活的抗炎作用。
The antipruritic effect of acupuncture was studied using a rat model of hindlimb scratching. After acupuncture or electroacupuncture (EA), which was conducted for 30 min, itch-associated behavior was induced by an intradermal injection of 2% serotonin (20 mu l) into the rostral back, and then numbers of scratching bouts were counted for 60 min. During the first experiment, acupuncture stimulations were applied to several different points. However acupuncture significantly reduced numbers of scratchings only when applied to cervical dermatomes. In the second experiment, plain acupuncture, or 2Hz, or 120Hz EA were applied to acupoints LI 11 and 1.14, at which acupuncture stimulation produced the greatest antipruritic effect in the 1st experiment, and as serotonin was administered in the same manner described for the 1st experiment. Results showed that 2Hz EA stimulation tended to increase pruritic bouts by approximately 18% versus the animals treated with plain acupuncture, whereas 120Hz EA stimulation tended to decrease pruritic bouts by approximately 39% compared with animals subjected to plain acupuncture. When nor-binaltorphimine (a kappa-opioid receptor antagonist) was pretreated to elucidate the relation between kappa-opioid receptor and the antipruritic effect of 120Hz EA, it was found to markedly inhibit the antipruritic effect of 120Hz EA. These results suggest that acupuncture and EA stimulation are effective treatments for pruritus if administered to dermatomes corresponding to affected sites or to adjacent dermatomes and that this effect is due to the antipruritic effect of kappa-opioid receptor activation maximally induced by high-frequency EA stimulation.