A G-protein-coupled neuropeptide Y-like receptor suppresses behavioral and sensory response to multiple stressful stimuli in Drosophila.
A G-protein-coupled neuropeptide Y-like receptor suppresses behavioral and sensory response to multiple stressful stimuli in Drosophila.
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DOI：
10.1523/jneurosci.3262-09.2010
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发表时间：
2010-02-17
期刊：
影响因子：
--
通讯作者：
Shen P
中科院分区：
文献类型：
--
作者：
Xu J;Li M;Shen P
最近的研究表明，人类NPY在应激反应和情绪管理中起着重要作用，在战斗暴露的退伍军人中观察到较高的NPY水平可能有助于应对创伤后应激。神经肽F （NPF）与人类神经肽Y （NPY）在黑腹龙体内的对应物，也显示出平行的活动，包括促进对各种应激源的恢复能力和预防不受控制的攻击行为。然而，目前尚不清楚NPY家族肽如何调节对各种压力源的身体和情绪反应。本研究表明，g蛋白偶联NPF受体NPFR1对蝇类和哺乳动物TRP家族不同亚型通道介导的幼虫对各种应激刺激的厌恶产生抑制作用。对幼虫感觉神经元和培养的人细胞的成像分析表明，NPFR1可以减弱由苍蝇TRPA和大鼠TRPV1通道介导的Ca2+内流。我们的研究结果表明，保守的NPF/NPFR1系统抑制TRP通道介导的神经兴奋可能是实现其广泛抗应激功能的主要机制。
Recent studies suggest that human NPY plays a prominent role in management of stress response and emotion, and higher NPY levels observed in combat-exposed veterans may help coping with post-traumatic stress. Neuropeptide F (NPF), the counterpart of human neuropeptide Y (NPY) in D. melanogaster, also displays parallel activities including promotion of resilience to diverse stressors and prevention of uncontrolled aggressive behavior. However, it remains unclear how NPY family peptides modulate physical and emotional responses to various stressors. Here we show that NPFR1, a G-protein coupled NPF receptor, exerts an inhibitory effect on larval aversion to diverse stressful stimuli mediated by different subtypes of fly and mammalian TRP family channels. Imaging analysis in larval sensory neurons and cultured human cells showed that NPFR1 attenuates Ca2+ influx mediated by fly TRPA and rat TRPV1 channels. Our findings suggest that suppression of TRP channel-mediated neural excitation by the conserved NPF/NPFR1 system may be a major mechanism for attaining its broad anti-stress function.