THEORETICAL TREATMENT OF DIFFUSION AND KINETICS IN AMPEROMETRIC IMMOBILIZED ENZYME ELECTRODES .1. REDOX MEDIATOR ENTRAPPED WITHIN THE FILM
THEORETICAL TREATMENT OF DIFFUSION AND KINETICS IN AMPEROMETRIC IMMOBILIZED ENZYME ELECTRODES .1. REDOX MEDIATOR ENTRAPPED WITHIN THE FILM
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DOI：
10.1016/0022-0728(95)04236-7
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发表时间：
1995-11-01
影响因子：
4.5
通讯作者：
PRATT, KFE
中科院分区：
文献类型：
--
作者：
BARTLETT, PN;PRATT, KFE
提出了一种在电极表面含有固定化酶和介体的均匀膜内扩散和反应的综合处理方法。将近似解析解与松弛法结合网格点自动分配的数值模拟结果进行了比较，发现两者吻合较好。用例图说明了不同极限情况之间的相互关系。给出了每种极限情况下电流的解析表达式，讨论了系统的行为，并识别了限速过程。电流与膜厚、酶负载量、介体负载量和底物浓度的关系是一种有用的诊断方法。该模型适用于研究葡萄糖氧化酶被包裹在氧化还原水凝胶膜中或固定在含有静电包裹介体的聚电解质膜中的体系。
A comprehensive treatment of the diffusion and reaction within a uniform film containing immobilized enzyme and mediator at an electrode surface is presented. Approximate analytical solutions and the results of numerical simulations using the relaxation method with automatic grid point allocation are compared and found to be in excellent agreement. Case diagrams are presented showing the interrelation between the different limiting cases. Analytical expressions for the current in each limiting case are given, the behaviour of the system is discussed, and the rate-limiting processes are identified. The potential dependence of the current is shown to be a useful diagnostic together with variation in film thickness, enzyme loading, mediator loading and substrate concentration.The model is appropriate to the study of systems such as glucose oxidase entrapped within a redox hydrogel film or immobilized within a polyelectrolyte membrane containing an electrostatically entrapped mediator.