Heparin-Mimicking Multilayer Coating on Polymeric Membrane via LbL Assembly of Cyclodextrin-Based Supramolecules
Heparin-Mimicking Multilayer Coating on Polymeric Membrane via LbL Assembly of Cyclodextrin-Based Supramolecules
复制标题
通过基于环糊精的超分子的 LbL 组装在聚合物膜上模拟肝素多层涂层
DOI：
10.1021/am506249r
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发表时间：
2014-12-20
影响因子：
9.5
通讯作者：
Zhao, Changsheng
中科院分区：
文献类型：
--
作者：
Deng, Jie;Liu, Xinyue;Zhao, Changsheng
在这项研究中，多功能和肝素模仿星形超分子沉积的3D多孔多层膜具有改善的生物相容性，通过逐层（LbL）的自组装方法在聚合物膜基板上制造。首先通过原子转移自由基聚合（ATRP）从基于β-环糊精（β-CD）的核合成星形肝素模拟聚阴离子（包括聚（苯乙烯磺酸盐-co-丙烯酸钠; Star-PSS-AANa）和聚（苯乙烯磺酸盐-co-聚（乙二醇）甲基醚甲基丙烯酸酯; Star-PSS-EGMA））和聚阳离子（聚（氯甲烷-季铵化的2-（二甲氨基）乙基甲基丙烯酸酯; Star-PMeDMA）。然后通过静电相互作用交替沉积聚阴离子和聚阳离子，在聚合物膜表面组装三维多孔多层膜。系统地研究了表面形貌和组成、水接触角、血液活化、血栓形成能力以及细胞活力。表面ATR-FTIR光谱和XPS光谱的结果证实了星形超分子在生物医学膜表面的成功沉积;扫描电子显微镜（SEM）和原子力显微镜（AFM）观察表明，修饰后的基底具有三维多孔表面形貌，这可能对生物界面产生很大的生物学影响。此外，系统的体外研究蛋白质吸附，血小板粘附，人血小板因子4，（PF 4，表示血小板活化）、活化部分凝血活酶时间（APTT）、凝血酶时间（TT）、凝血活化（凝血酶-抗凝血酶III复合物（达特，表示血液凝固剂））和血液相关的补体激活（C3 a和C5 a，表示炎症可能性）证实，肝素模拟多层涂层膜表现出超低的血液组分活化和优异的血液相容性。同时，表面包覆后，内皮细胞的活力也得到了提高，这表明，肝素模拟多层涂层可能会扩大聚合物膜在生物医学领域的应用领域。
In this study, multifunctional and heparin-mimicking star-shaped supramolecules-deposited 3D porous multilayer films with improved biocompatibility were fabricated via a layer-by-layer (LbL) self-assembly method on polymeric membrane substrates. Star-shaped heparin-mimicking polyanions (including poly(styrenesulfonate-co-sodium acrylate; Star-PSS-AANa) and poly(styrenesulfonate-co-poly(ethylene glycol)methyl ether methacrylate; Star-PSS-EGMA)) and polycations (poly(methyl chloride-quaternized 2-(dimethylamino)ethyl methacrylate; Star-PMeDMA) were first synthesized by atom transfer radical polymerization (ATRP) from beta-cyclodextrin (beta-CD) based cores. Then assembly of 3D porous multilayers onto polymeric membrane surfaces was carried out by alternating deposition of the polyanions and polycations via electrostatic interaction. The surface morphology and composition, water contact angle, blood activation, and thrombotic potential as well as cell viability for the coated heparin-mimicking films were systematically investigated. The results of surface ATR-FTIR spectra and XPS spectra verified successful deposition of the star-shaped supramolecules onto the biomedical membrane surfaces; scanning electron microscopy (SEM) and atomic force microscopy (AFM) observations revealed that the modified substrate had 3D porous surface morphology, which might have a great biological influence on the biointerface. Furthermore, systematic in vitro investigation of protein adsorption, platelet adhesion, human platelet factor 4 (PF4, indicates platelet activation), activate partial thromboplastin time (APTT), thrombin time (TT), coagulation activation (thrombin-antithrombin III complex (TAT, indicates blood coagulant)), and blood-related complement activation (C3a and C5a, indicates inflammation potential) confirmed that the heparin-mimicking multilayer coated membranes exhibited ultralow blood component activations and excellent hemocompatibility. Meanwhile, after surface coating, endothelial cell viability was also promoted, which indicated that the heparin-mimicking multilayer coating might extend the application fields of polymeric membranes in biomedical fields.