Population Dynamics of Phenol-Degrading Bacteria in Activated Sludge Determined by gyrB-Targeted Quantitative PCR
Population Dynamics of Phenol-Degrading Bacteria in Activated Sludge Determined by gyrB-Targeted Quantitative PCR
复制标题
gyrB 靶向定量 PCR 测定活性污泥中苯酚降解细菌的种群动态
DOI：
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发表时间：
1998
影响因子：
4.4
通讯作者：
S. Harayama
中科院分区：
文献类型：
--
作者：
Kazuya Watanabe;Satoshi Yamamoto;S. Hino;S. Harayama
摘要描述了一种用于定量引入活性污泥微生物群落的细菌种群的方法。该方法包括从活性污泥中提取DNA，用从非引入的活性污泥中提取的DNA适当稀释提取的DNA，用一组菌株特异性引物从引入的菌株中PCR扩增gyrB基因片段，并通过光密度法定量PCR产物。通过分析两种苯酚降解细菌，恶臭假单胞菌BH和丛毛单胞菌属菌株E6，已被引入到苯酚消化活性污泥的人口动态的方法的充分性进行了检查。在引入后立即通过PCR方法测定的两个种群中的每一个的密度与根据接种物的平板计数估计的密度一致。该定量PCR方法揭示了不同苯酚负荷条件下活性污泥中两种菌株的不同种群动态。这两种菌株在活性污泥中的行为反映了在实验室无菌培养中测定的菌株的生长动力学。
ABSTRACT A method for quantifying bacterial populations introduced into an activated-sludge microbial community is described. The method involves extraction of DNA from activated sludge, appropriate dilution of the extracted DNA with DNA extracted from nonintroduced activated sludge, PCR amplification of a gyrB gene fragment from the introduced strain with a set of strain-specific primers, and quantification of the electrophoresed PCR product by densitometry. The adequacy of the method was examined by analyzing the population dynamics of two phenol-degrading bacteria, Pseudomonas putida BH and Comamonas sp. strain E6, that had been introduced into phenol-digesting activated sludge. The density of each of the two populations determined by the PCR method immediately after the introduction was consistent with the density estimated from a plate count of the inoculum. This quantitative PCR method revealed different population dynamics for the two strains in the activated sludge under different phenol-loading conditions. The behavior of both of these strains in the activated sludge reflected the growth kinetics of the strains determined in laboratory axenic cultures.
影响因子：
56.9
作者：
DELONG, EF;WICKHAM, GS;PACE, NR
通讯作者：
PACE, NR