Characterisation of a Helicobacter pylori phage (HP1).

Characterisation of a Helicobacter pylori phage (HP1).
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幽门螺杆菌噬菌体 (HP1) 的表征。

DOI:
10.1099/00222615-38-4-245
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发表时间:
1993
影响因子:
3
通讯作者:
E. Schmid
E. Schmid
中科院分区:
医学3区
文献类型:
--
作者:
E. H. Heinegg;H. P. Nalik;E. Schmid

文献摘要

被引文献

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用溶原性幽门螺杆菌的含噬菌体上清液感染两株幽门螺杆菌。pylori菌株IMMi 290/89导致噬菌体HP 1的裂解循环和增殖。在阴性染色的制备物中,空噬菌体头部的直径测量为55-60 nm,成熟头部的直径测量为50 nm。柔性的、有条纹的噬菌体尾是c。长170 nm,直径9.5 nm。该噬菌体在蔗糖密度梯度中的平均密度为1.40 g/cm ~ 3,含有双链DNA c。22,000 bp长。
The infection of two Helicobacter pylori strains with a phage-containing supernate of the lysogenic H. pylori strain IMMi 290/89 resulted in a lytic cycle and propagation of phage HP1. In negatively-stained preparations, the empty phage heads measured 55-60 nm in diameter and mature heads measured 50 nm. The flexible, striated phage tail was c. 170 nm in length and 9.5 nm in diameter. The phage showed a mean density of 1.40 g/cm3 in sucrose-density gradients and contained double-stranded DNA c. 22,000 bp in length.