Maize Glossy2 and Glossy2-like Genes Have Overlapping and Distinct Functions in Cuticular Lipid Deposition1[OPEN]
Maize Glossy2 and Glossy2-like Genes Have Overlapping and Distinct Functions in Cuticular Lipid Deposition1[OPEN]
复制标题
DOI：
10.1104/pp.20.00241
复制
发表时间：
2020-07-01
期刊：
影响因子：
7.4
通讯作者：
Nikolau, Basil J.
中科院分区：
文献类型：
--
作者：
Alexander, Liza Esther;Okazaki, Yozo;Nikolau, Basil J.
玉米Glossy 2和Glossy 2-like基因在角质层脂质沉积中的功能作用的转基因解剖揭示了共同和不同的functions.Plant表皮细胞表达独特的分子机制，并置细胞内脂质成分和单向分泌到植物表面的独特的细胞外角质层脂质的组装。在玉米（Zea mays）中，glossy 2（gl 2）位点的突变影响细胞外表皮脂质的沉积。基于序列的基因组扫描在玉米基因组中发现了一个新的Gl 2同源物，命名为Gl 2-like。Gl 2-like和Gl 2基因都是BAHD酰基转移酶超家族的成员，与拟南芥（Arabidopsis thaliana）的CER 2基因序列相似。转基因实验表明Gl 2-like和Gl 2在功能上补充了突变体Glasopsiser 2，对植物的表皮脂质和脂质组具有不同的影响，特别是影响较长的烷基链酰基脂质，特别是在32-碳链长度处。对BAHD催化基序HXXXDX的定点突变表明，Gl 2-like需要这种催化能力才能完全互补cer 2的功能，但Gl 2可以在不需要这种催化基序的情况下完成互补。这些发现表明Gl 2和Gl 2-like在它们的表皮脂质功能上是重叠的，但在进化上已经分化为获得非重叠的功能。
Transgenesis dissection of the functional roles of maize Glossy2 and Glossy2-like genes in cuticular lipid deposition reveals both common and distinct functions.Plant epidermal cells express unique molecular machinery that juxtapose the assembly of intracellular lipid components and the unique extracellular cuticular lipids that are unidirectionally secreted to plant surfaces. In maize (Zea mays), mutations at theglossy2 (gl2)locus affect the deposition of extracellular cuticular lipids. Sequence-based genome scanning identified a newGl2homolog in the maize genome, namelyGl2-like. Both theGl2-likeandGl2genes are members of the BAHD superfamily of acyltransferases, with close sequence similarity to the Arabidopsis (Arabidopsis thaliana)CER2gene. Transgenic experiments demonstrated thatGl2-likeandGl2functionally complement the Arabidopsiscer2mutation, with differential influences on the cuticular lipids and the lipidome of the plant, particularly affecting the longer alkyl chain acyl lipids, especially at the 32-carbon chain length. Site-directed mutagenesis of the putative BAHD catalytic HXXXDX-motif indicated thatGl2-likerequires this catalytic capability to fully complement thecer2function, butGl2can accomplish complementation without the need for this catalytic motif. These findings demonstrate thatGl2andGl2-likeoverlap in their cuticular lipid function, but have evolutionarily diverged to acquire nonoverlapping functions.