Superficieibacter electus gen. nov., sp. nov., an Extended-Spectrum β-Lactamase Possessing Member of the Enterobacteriaceae Family, Isolated From Intensive Care Unit Surfaces.
Superficieibacter electus gen. nov., sp. nov., an Extended-Spectrum β-Lactamase Possessing Member of the Enterobacteriaceae Family, Isolated From Intensive Care Unit Surfaces.
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DOI：
10.3389/fmicb.2018.01629
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发表时间：
2018
影响因子：
5.2
通讯作者：
Dantas G
中科院分区：
文献类型：
--
作者：
Potter RF;D'Souza AW;Wallace MA;Shupe A;Patel S;Gul D;Kwon JH;Beatty W;Andleeb S;Burnham CD;Dantas G
在巴基斯坦的一项纵向调查中，从两个不同的重症监护病房表面回收了两种革兰氏阴性杆菌菌株，命名为BP-1（T）和BP-2。使用bioMerieux VITEK MS IVD v2.3.3和Bruker BioTyper MALDI-TOF质谱平台鉴别了两种菌株。为了更精确地确定BP-1（T）和BP-2的分类学身份，我们采用了生物化学和微卫星基因组学方法。菌株BP-1（T）的16 S rRNA基因序列与柠檬酸杆菌CDC 2991-81（T）（98.63%）、柠檬酸杆菌CECT 863（T）（98.56%）、塞氏柠檬酸杆菌NBRC 105722（T）（97.74%）和啮齿类柠檬酸杆菌NBRC 105723（T）（97.74%）的同源性最高。使用肠杆菌科的分析谱指数（API 20 E）的BP-1（T）的生化利用方案表明其酶功能在肠杆菌科中是独特的，但最接近于克鲁维拉属，阴沟肠杆菌和科氏柠檬酸杆菌/法默氏柠檬酸杆菌。BP-1（T）和BP-2与克鲁维拉菌属、柠檬酸杆菌属、埃希氏菌属、沙门氏菌属、科萨孔菌属、Siccibacter属和志贺菌属的模式菌株之间的共有基因的系统基因组学分析表明，BP-1（T）和BP-2分离物与这些属形成不同的分支。平均核苷酸同一性分析表明BP-1（T）和BP-2是相同的物种。生物化学和细菌基因组分析表明菌株BP-1（T）和BP-2代表来自肠杆菌科新属的新物种，其名称为Superficieibacter electus gen. nov.，新种，本文提出模式菌株为BP-1（T）（= ATCC BAA-2937，= NBRC 113412）。
Two Gram-negative bacilli strains, designated BP-1(T) and BP-2, were recovered from two different Intensive Care Unit surfaces during a longitudinal survey in Pakistan. Both strains were unidentified using the bioMerieux VITEK MS IVD v2.3.3 and Bruker BioTyper MALDI-TOF mass spectrometry platforms. To more precisely determine the taxonomic identity of BP-1(T) and BP-2, we employed a biochemical and phylogenomic approach. The 16S rRNA gene sequence of strain BP-1(T) had the highest identity to Citrobacter farmeri CDC 2991-81(T) (98.63%) Citrobacter amalonaticus CECT 863(T) (98.56%), Citrobacter sedlakii NBRC 105722(T) (97.74%) and Citrobacter rodentium NBRC 105723(T) (97.74%). The biochemical utilization scheme of BP-1(T) using the Analytic Profile Index for Enterobacteriaceae (API20E) indicated its enzymatic functions are unique within the Enterobacteriaceae but most closely resemble Kluyvera spp., Enterobacter cloacae and Citrobacter koseri/farmeri. Phylogenomic analysis of the shared genes between BP-1(T), BP-2 and type strains from Kluyvera, Citrobacter, Escherichia, Salmonella, Kosakonia, Siccibacter and Shigella indicate that BP-1(T) and BP-2 isolates form a distinct branch from these genera. Average Nucleotide Identity analysis indicates that BP-1(T) and BP-2 are the same species. The biochemical and phylogenomic analysis indicate strains BP-1(T) and BP-2 represent a novel species from a new genus within the Enterobacteriaceae family, for which the name Superficieibacter electus gen. nov., sp. nov., is proposed. The type strain is BP-1(T) (= ATCC BAA-2937, = NBRC 113412).