Apoptotic index and bcl-2 expression as prognostic factors in colorectal carcinoma
Apoptotic index and bcl-2 expression as prognostic factors in colorectal carcinoma
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DOI：
10.1159/000070304
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发表时间：
2003-01-01
期刊：
影响因子：
3.5
通讯作者：
Pyrhönen, S
中科院分区：
文献类型：
--
作者：
Bendardaf, R;Ristamäki, R;Pyrhönen, S
目的：检查 57 例结直肠癌（49 例原发肿瘤和 8 例转移瘤）中的程序性细胞死亡，并确定细胞凋亡在结直肠癌中的预后意义。方法：采用末端脱氧核苷酸转移酶介导的地高辛缺口末端标记（Tunel 法），通过计数凋亡小体确定细胞凋亡指数（Al），并采用免疫组化法检测 bcl-2 的表达。采用统计分析检验临床变量、组织病理学数据、Al和bcl-2表达在预测这些患者临床结果中的价值，并使用Kaplan-Meier法计算生存函数。结果：发现 Al 对生存具有显着的独立影响 (p = 0.0006)，Al 值越低，生存率越高。结论：总而言之，这些发现表明铝是结直肠癌的一个有用的预后因素。
Objectives: To examine programmed cell death in 57 colorectal carcinomas (49 primary tumours and 8 metastases) and determine the prognostic significance of apoptosis in colorectal cancer. Methods: Apoptotic index (Al) was ascertained by counting apoptotic bodies, using terminal deoxynucleotidyl transferase mediated digoxigenin nick end labelling (Tunel assay) and the expression of bcl-2 was examined immunohistochemically. Statistical analysis was used to test the value of clinical variables, histopathological data, Al and bcl-2 expression in predicting the clinical outcome of these patients and the survival function was calculated using the Kaplan-Meier method. Results: Al was found to have a significant independent effect on survival (p = 0.0006), with lower values of Al conveying better survival. Conclusion: In summary, these findings reveal that Al is a useful prognostic factor in colorectal carcinoma.