Characterization of the in vitro effects of 5-hydroxytryptamine (5-HT) on identified neurones of the rat dorsal motor nucleus of the vagus (DMV)
Characterization of the in vitro effects of 5-hydroxytryptamine (5-HT) on identified neurones of the rat dorsal motor nucleus of the vagus (DMV)
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DOI：
10.1038/sj.bjp.0702908
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发表时间：
1999-11-01
影响因子：
7.3
通讯作者：
Travagli, RA
中科院分区：
文献类型：
--
作者：
Browning, KN;Travagli, RA
1在脑干薄片上应用全细胞膜片钳技术研究5-羟色胺（5-HT）对胃迷走神经运动背核（DMV）投射神经元的影响。2肠神经元（69%）比胃神经元（47%）对5-HT产生浓度依赖性内向电流，该电流可被5-HT 2A受体拮抗剂酮色林（1 μ M）完全拮抗。3刺激孤束核（NTS）诱导抑制性突触电流，通过应用5-HT 1A受体激动剂8-OHDPAT（1 μ M）或5-HT 1A/1B受体激动剂TFMPP（1 μ M）分别在61%和52%的胃和延髓投射神经元中降低幅度。5-HT还显著降低自发抑制电流的频率，但不降低其幅度。4这些数据表明，5-HT直接兴奋肠投射神经元的比例大于胃投射神经元，并抑制从NTS到DMV的突触传递。这些数据意味着DMV神经元对5-HT的反应可以通过其特定的投射来确定和分类。
1 Whole cell patch clamp techniques were used on thin brainstem slices to investigate the effects of 5-hydroxytryptamine (5-HT) on gastrointestinal-projecting dorsal motor nucleus of the vagus (DMV) neurones Neurones were identified as projecting to the stomach (n=122) or intestine (n = 84) if they contained the fluorescent tracer Dil after it had been applied to the gastric fundus, corpus or antrum/pylorus or to the duodenum or caecum.2 A higher proportion of intestinal neurones (69%) than gastric neurones (47%) responded to 5-HT with a concentration-dependent inward current which was antagonized fully by the 5-HT2A receptor antagonist ketanserin (1 mu M).3 Stimulation of the nucleus tractus solitarius (NTS) induced inhibitory synaptic currents that were reduced in amplitude by application of the 5-HT1A receptor agonist 8-OHDPAT (1 mu M) or the 5-HT1A/1B receptor agonist TFMPP (1 mu M) in 61% and 52% of gastric- and intestinal-projecting neurones, respectively. 5-HT also significantly reduced the frequency but not the amplitude of spontaneous inhibitory currents.4 These data show that 5-HT excites directly a larger proportion of intestinal projecting neurones than gastric-projecting neurones, as well as inhibiting synaptic transmission from the NTS to the DMV. These data imply that the response to DMV neurones to 5-HT may be determined and classified by their specific projections.