DNA complexed structure of the key transcription factor initiating development in sporulating bacteria.
DNA complexed structure of the key transcription factor initiating development in sporulating bacteria.
复制标题
DNA 复合了启动孢子细菌发育的关键转录因子的结构。
DOI：
10.1016/s0969-2126(02)00803-1
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Varughese,KottayilI
中科院分区：
文献类型：
--
作者：
Zhao,Haiyan;Msadek,Tarek;Zapf,James;Madhusudan;Hoch,JamesA;Varughese,KottayilI
芽孢杆菌的孢子形成是细菌的最终适应性反应，需要精确协调表达复杂的遗传途径，并通过磷酸化形式Spo0A（一种多效性反应调节转录因子）的积累而启动。Spo0A控制所有芽孢杆菌中数百个基因的转录，包括病原体如炭疽杆菌中的芽孢形成和毒素调节基因。测定了枯草杆菌蛋白酶复合物Spo0A效应结构域与其DNA靶的晶体结构。在晶格中，两个分子在结合到DNA上的相邻位点时形成串联二聚体。晶体中揭示的蛋白质：蛋白质和蛋白质：DNA界面为解释转录激活过程和设计对抗这些细菌感染的药物提供了基础。
Sporulation inBacillusspecies, the ultimate bacterial adaptive response, requires the precisely coordinated expression of a complex genetic pathway, and is initiated through the accumulation of the phosphorylated form of Spo0A, a pleiotropic response regulator transcription factor. Spo0A controls the transcription of several hundred genes in all spore-formingBacilliincluding genes for sporulation and toxin regulation in pathogens such asBacillus anthracis. The crystal structure of the effector domain of Spo0A fromBacillus subtilisin complex with its DNA target was determined. In the crystal lattice, two molecules form a tandem dimer upon binding to adjacent sites on DNA. The protein:protein and protein:DNA interfaces revealed in the crystal provide a basis for interpreting the transcription activation process and for the design of drugs to counter infections by these bacteria.