THE IDENTIFICATION, ORIGIN, AND MIGRATION OF THE PRIMORDIAL GERM CELLS IN THE MOUSE EMBRYO
THE IDENTIFICATION, ORIGIN, AND MIGRATION OF THE PRIMORDIAL GERM CELLS IN THE MOUSE EMBRYO
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DOI：
10.1002/ar.1091180202
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发表时间：
1954-01-01
影响因子：
--
通讯作者：
CHIQUOINE, AD
中科院分区：
文献类型：
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作者：
CHIQUOINE, AD
发现原始生殖细胞具有如此高程度的碱性磷酸酶活性，如 所示。 Gomori (1946) 的修订方法表明，可以在较短的孵育时间内几乎选择性地对细胞进行染色，并追踪它们从卵黄囊到生发嵴的迁移。在交配龄 6 至 12 天的胚胎的连续切片中研究了生殖细胞的分布。第8天观察到生殖细胞，散在原条尾端、尿囊中胚层根部及邻近卵黄囊细胞中。据信生殖细胞可能起源于卵黄囊内脏壁。 9至12天胚胎中的细胞随后沿着开放的中肠和尿囊柄迁移到后肠，然后在背侧肠系膜内向背侧迁移，以达到它们在生发嵴中的最终位置。生殖细胞高酶活性的意义尚不清楚，但这并不是所有迁移胚胎细胞的特征。
Primordial germ cells were found to possess such a high degree of alkaline phosphatase activity as demonstrated by the . revised method of Gomori (1946) that it was possible with short incubation periods to stain the cells almost selectively and to trace their migration from the yolk sac to the germinal ridge. Distribution of germ cells was studied in. serial sections of embryos from 6 to 12 days'' copulation age. Germ cells were observed on the 8th day, scattered among the cells of the caudal end of the primitive streak, the root of the allantoic mesoderm, and the adjacent yolk sac. It is believed that germ cells probably originate in the yolk sac splanchnopleure. Later migration of the cells in 9- to 12_day embryos is along the open mid gut and stalk of the allantois to the hind gut and then dorsally within the dorsal mesentery to attain their final position in the germinal ridges. Significance of the high enzymatic activity of the germ cells is not known but it is not characteristic of all migrating embryonic cells.