Protective immunity induced by Eimeria common antigen 14-3-3 against Eimeria tenella, Eimeria acervulina and Eimeria maxima.
Protective immunity induced by Eimeria common antigen 14-3-3 against Eimeria tenella, Eimeria acervulina and Eimeria maxima.
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艾美耳球虫共同抗原14-3-3诱导的针对柔嫩艾美耳球虫、弯型艾美耳球虫和巨型艾美耳球虫的保护性免疫
DOI：
10.1186/s12917-018-1665-z
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发表时间：
2018-11-12
影响因子：
2.6
通讯作者：
Song X
中科院分区：
文献类型：
--
作者：
Liu J;Liu L;Li L;Tian D;Li W;Xu L;Yan R;Li X;Song X
禽球虫病通常是由世界范围内的几种艾美耳球虫共同感染引起的。开发具有多个艾美耳球虫物种共同抗原的多价疫苗是控制艾美耳球虫临床常见合并感染的有希望的策略。14-3-3是大肠杆菌的免疫原性共同抗原之一。tenella、E. acervulina和E.马克西姆本研究的目的是评估Ea 14 -3-3以DNA疫苗形式单独和同时对抗三种艾美耳球虫感染的免疫原性和保护功效。用pVAX-Ea 14 -3-3免疫小鼠后，Ea 14 -3-3基因在肌肉中转录并表达。结果表明，pVAX-Ea 14 -3-3免疫鸡后，CD 4+和CD 8 + T淋巴细胞比例显著增加，并产生了较强的IgY免疫应答。类似地，pVAX-Ea 14 -3-3刺激鸡的脾细胞产生高水平的Th 1型（IFN-γ、IL-2）和Th 2型（IL-4）细胞因子。与对照组相比，疫苗诱导的免疫应答可显著增加免疫鸡的增重，降低卵囊产量，减轻肠道病变，并诱导中等程度的抗球虫指数（ACI）。这些结果表明Ea 14 -3-3是高度免疫原性的并且能够诱导显著的免疫应答。此外，Ea 14 -3-3抗原可以提供针对柔嫩艾美耳球虫、堆型艾美耳球虫、巨型艾美耳球虫单独以及与三种艾美耳球虫的组合的感染的有效保护。本研究的重要结果为开发自然条件下不同种属艾美耳球虫混合感染的多价艾美耳球虫疫苗提供了有效的候选抗原。本文的在线版本（10.1186/s12917-018-1665-z）包含补充材料，可供授权用户使用。
Avian coccidiosis is often caused by co-infection with several species of Eimeria worldwide. Developing a multivalent vaccine with an antigen common to multiple Eimeria species is a promising strategy for controlling clinical common co-infection of Eimeria. In the previous study, 14–3-3 was identified as one of the immunogenic common antigen in E. tenella, E. acervulina and E. maxima. The aim of the present study was to evaluate the immunogenicity and protective efficacy of Ea14–3-3 in the form of DNA vaccine against infection with three species of Eimeria both individually and simultaneously. After vaccination with pVAX-Ea14–3-3, the Ea14–3-3 gene was transcribed and expressed in the injected muscles. Vaccination with pVAX-Ea14–3-3 significantly increased the proportion of CD4+ and CD8+ T lymphocytes and produced a strong IgY response in immunized chickens. Similarly, pVAX-Ea14–3-3 stimulated the chicken’s splenocytes to produce high levels of Th1-type (IFN-γ, IL-2) and Th2-type (IL-4) cytokines. The vaccine-induced immune response was responsible to increase weight gain, decreased the oocyst output, and alleviated enteric lesions significantly in immunized chickens as compared to control group, in addition to induce moderate anti-coccidial index (ACI). These results indicate that Ea14–3-3 is highly immunogenic and capable to induce significant immune responses. Furthermore, Ea14–3-3 antigen can provide effective protection against infection with Eimeria tenella, Eimeria acervulina, Eimeria maxima both individually and in combination with three Eimeria species. Significant outcomes of our study provide an effective candidate antigen for developing a multivalent Eimeria vaccine against mixed infection with various Eimeria species under natural conditions. The online version of this article (10.1186/s12917-018-1665-z) contains supplementary material, which is available to authorized users.
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影响因子：
4.8
作者：
Lalle, Marco;Curra, Chiara;Ponzi, Marta
通讯作者：
Ponzi, Marta
影响因子：
2.6
作者：
Holdsworth, PA;Conway, DP;Williams, RB
通讯作者：
Williams, RB
影响因子：
1.3
作者：
MCMANUS, EC;CAMPBELL, WC;CUCKLER, AC
通讯作者：
CUCKLER, AC
DOI：
10.1186/1479-0556-2-17
发表时间：
2004-12-03
期刊：
Genetic vaccines and therapy
影响因子：
--
作者：
Ivory C;Chadee K
通讯作者：
Chadee K
影响因子：
3.1
作者：
del Cacho, Emilio;Gallego, Margarita;Sanchez-Acedo, Caridad
通讯作者：
Sanchez-Acedo, Caridad