Rapid diagnosis of Hemophilus influenzae type b infections by latex particle agglutination and counterimmunoelectrophoresis.
Rapid diagnosis of Hemophilus influenzae type b infections by latex particle agglutination and counterimmunoelectrophoresis.
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通过乳胶颗粒凝集和反免疫电泳快速诊断 b 型流感嗜血杆菌感染。
DOI：
10.1016/s0022-3476(78)80596-4
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发表时间：
1978
期刊：
影响因子：
--
通讯作者：
D. Smith
中科院分区：
文献类型：
--
作者：
J. Ward;G. Siber;D. Scheifele;D. Smith
在一项临床试验中比较了乳胶颗粒凝集和反免疫电泳技术，以证明它们在侵袭性流感嗜血杆菌B型疾病快速诊断中的敏感性、特异性和实用性。LPA（由内务人员进行的简化LPA）和本研究中使用的CIE分别检测到0.2、0.5和1至10 mg/ml的Hib荚膜抗原。通过这些检测方法对106例疑似由Hib引起的疾病进行了前瞻性评价。其中39例通过培养或抗原检测或两者确认。LPA和简化LPA对Hib的诊断敏感性和特异性均高于CIE（P<0.01），尤其是对非脑膜炎的侵袭性疾病。LPA价格便宜，可以快速进行，并检测所有侵入性Hib感染。结果强调抗原检测在Hib感染的快速诊断中的有用性，证明LPA比CIE更敏感和特异，并且可以由医生方便地进行。
Latex particle agglutination and counterimmunoelectrophoresis techniques were compared in a clinical trial to demonstrate their sensitivity, specificity, and usefulness in the rapid diagnosis of invasiveHemophilus influenzaetype b disease. LPA, a simplified LPA performed by house officers, and CIE used in this study detected 0.2, 0.5, and 1 to 10 mg/ml of Hib capsular antigen, respectively. One hundred-six illnesses suspected of being caused by Hib were evaluated prospectively by these assays. A total of 39 of these were confirmed by culture or detection of antigen or both. LPA and simplified LPA were more sensitive and specific than CIE in the diagnosis of Hib disease (P<0.01), especially in invasive disease other than meningitis. LPA is inexpensive, can be performed quickly, and detects all invasive Hib infections. The results emphasize the usefulness of antigen detection in the rapid diagnosis of Hib infections, demonstrate that LPA is more sensitive and specific than CIE, and can be conveniently performed by physicians.