Differential expression profiles of long non-coding RNAs reveal potential biomarkers for identification of human gastric cancer
Differential expression profiles of long non-coding RNAs reveal potential biomarkers for identification of human gastric cancer
复制标题
长非编码RNA的差异表达谱揭示了鉴定人胃癌的潜在生物标志物
DOI：
10.3892/or.2015.4531
复制
发表时间：
2016-03-01
期刊：
影响因子：
4.2
通讯作者：
Pu, Yuepu
中科院分区：
文献类型：
--
作者：
Li, Chengyun;Liang, Geyu;Pu, Yuepu
胃癌是世界范围内致死率最高的恶性肿瘤之一。为了降低其高死亡率，迫切需要敏感和特异的早期检测生物标志物。近年来研究发现，肿瘤特异性长链非编码RNA（lncRNA）可能成为肿瘤早期诊断和治疗的潜在生物标志物。在本研究中，lncRNA和mRNA表达谱的GC标本及其配对的相邻的非癌组织进行。通过微阵列分析鉴定差异表达的lncRNA和mRNA。通过基因本体论和通路分析确定差异mRNA的功能，通过构建共表达网络研究lncRNA的功能，寻找其与相应mRNA的关系。我们将共表达网络、mRNA功能和微阵列谱差异表达的结果联系起来，选择了14个显着差异表达的关键lncRNA和21个关键mRNA。在50例新诊断的GC患者中进行定量RT-PCR（qRT-PCR）以验证这些关键RNA。数据显示，RP 5 - 919 F19、CTD-2541 M15和UCA 1的表达显著更高。AP 000459、LOC 101928316、RP 11 - 167 N4和LINC 01071在30例进展期胃癌组织中的表达显著低于癌旁组织，P
Gastric cancer (GC) is one of the most lethal malignancies worldwide. To reduce its high mortality, sensitive and specific biomarkers for early detection are urgently needed. Recent studies have reported that tumor-specific long non-coding RNAs (lncRNAs) seem to be potential biomarkers for the early diagnosis and treatment of cancer. In the present study, lncRNA and mRNA expression profiling of GC specimens and their paired adjacent non-cancerous tissues was performed. Differentially expressed lncRNAs and mRNAs were identified through microarray analysis. The function of differential mRNA was determined by gene ontology and pathway analysis and the functions of lncRNAs were studied by constructing a co-expression network to find the relationships with corresponding mRNAs. We connected the co-expression network, mRNA functions, and the results of the microarray profile differential expression and selected 14 significantly differentially expressed key lncRNAs and 21 key mRNAs. Quantitative RT-PCR (qRT-PCR) was conducted to verify these key RNAs in 50 newly diagnosed GC patients. The data showed that RP5-919F19, CTD-2541M15 and UCA1 was significantly higher expressed. AP000459, LOC101928316, RP11-167N4 and LINC01071 expression was significantly lower in 30 advanced GC tumor tissues than adjacent non-tumor tissues P