Opioid propeptide mRNA content and receptor density in the brains of AA and ANA rats
Opioid propeptide mRNA content and receptor density in the brains of AA and ANA rats
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DOI：
10.1016/s0024-3205(00)00517-8
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发表时间：
2000-04-07
期刊：
影响因子：
6.1
通讯作者：
Gianoulakis, C
中科院分区：
文献类型：
--
作者：
Marinelli, PW;Kiianmaa, K;Gianoulakis, C
最近的证据表明，乙醇摄入的奖励效应和内源性阿片活性之间的关联。本研究探讨存在的差异，阿片肽的mRNA含量和μ和κ阿片受体密度，乙醇幼稚AA和ANA大鼠选择性繁殖的高和低酒精消费，分别。原位杂交被用来比较不同的大脑区域的阿黑皮素原，前脑啡肽和强啡肽原mRNA的含量已知参与成瘾药物的增强性能，从每个线之间的大鼠。结果表明，AA大鼠下丘脑弓状核、前额叶皮质和丘脑背内侧核的阿黑皮素原、前脑啡肽原和强啡肽原mRNA的含量均显著增加（p <0.05）。受体放射自显影使用H-3标记的μ和κ阿片受体特异性配体进行。AA大鼠被发现有一个更大的密度μ阿片受体的壳区域的丘脑核和前额叶皮层，但较低的密度kappa阿片受体的腹内侧下丘脑，相比ANA大鼠。目前的数据表明，存在的内源性阿片类药物系统的不同成分的活动在一些大脑区域与奖励和强化的过程中的遗传差异，因此，可能发挥作用，在确定AA和ANA大鼠之间的乙醇饮用的差异。
Recent evidence has indicated an association between the rewarding effects of ethanol intake and endogenous opioid activity. The present studies examine the presence of differences in opioid peptide mRNA content and mu and kappa opioid receptor densities, between ethanol naive AA and ANA rats bred selectively for their high and low alcohol consumption, respectively. In situ hybridization was used to compare the content of proopiomelanocortin, proenkephalin and prodynorphin mRNA in distinct brain regions known to be involved in the reinforcing properties of addictive drugs, between rats from each line. Results indicated that AA rats had a significantly greater content of proopiomelanocortin mRNA in the arcuate nucleus of the hypothalamus, of proenkephalin mRNA in the prefrontal cortex and of prodynorphin mRNA in the mediodorsal nucleus of the thalamus (p less than or equal to .05). Receptor autoradiography was performed using H-3-labeled ligands specific for mu and kappa opioid receptors. AA rats were found to have a greater density of mu opioid receptors in the shell region of the nucleus accumbens and prefrontal cortex, but a lower density of kappa opioid receptors in the ventromedial hypothalamus, compared to ANA rats. The present data demonstrate the presence of inherited differences in the activity of distinct components of the endogenous opioid system in some brain regions associated with the processes of reward and reinforcement; and as such, may play a role in determining differences in ethanol drinking between AA and ANA rats.