Alterations of PLCβ1 in mouse eggs change calcium oscillatory behavior following fertilization
Alterations of PLCβ1 in mouse eggs change calcium oscillatory behavior following fertilization
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DOI：
10.1016/j.ydbio.2007.09.028
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发表时间：
2007-12-01
影响因子：
2.7
通讯作者：
Williams, Carnien J.
中科院分区：
文献类型：
--
作者：
Tgarashi, Hideki;Knott, Jason G.;Williams, Carnien J.
由磷脂酶C（PLC）作用产生的肌醇1，4，5-三磷酸介导细胞内Ca 2+的释放，这是精子诱导的哺乳动物卵子激活所必需的。尽管PLC zeta构成受精卵中PLC总量的一个非常小的部分，但目前很多注意力集中在精子来源的PLC在产生卵细胞内Ca 2+变化中的作用上。鸡蛋表达几种异构体的PLC，但在精子诱导的钙振荡的鸡蛋衍生的PLC的作用还没有被检查。通过转基因RNAi方法减少卵PLC β 1 I导致授精后Ca 2+瞬时振幅显著降低，但持续时间或频率没有降低。此外，通过显微注射Plc β 1 cRNA过表达PLC β 1显著干扰了Ca 2+瞬变的持续时间和频率，并破坏了第一瞬变的特征形状。这些结果提供了第一个证据的作用，鸡蛋衍生的PLC与精子衍生的PLC ζ在鸡蛋激活。(C)2007爱思唯尔公司All rights reserved.
Inositol 1,4,5-trisphosphate generated by the action of a phospholipase C (PLC) mediates release of intracellular Ca2+ that is essential for sperm-induced activation of mammalian eggs. Much attention currently focuses on the role of sperm-derived PLC in generating changes in egg intracellular Ca2+ despite the fact that PLC zeta constitutes a very small fraction of the total amount of PLC in a fertilized egg. Eggs express several isoforms of PLC, but a role for an egg-derived PLC in sperm-induced Ca2+ oscillations has not been examined. Reducing egg PLC beta 1 I by a transgenic RNAi approach resulted in a significant decrease in Ca2+ transient amplitude, but not duration or frequency, following insemination. Furthermore, overexpressing PLC beta 1 by microinjecting a Plc beta 1 cRNA significantly perturbed the duration and frequency of Ca2+ transients and disrupted the characteristic shape of the first transient. These results provide the first evidence for a role of an egg-derived PLC acting in conjunction with a sperm-derived PLC zeta in egg activation. (C) 2007 Elsevier Inc. All rights reserved.