Cell‐laden biphasic scaffolds with anisotropic structure for the regeneration of osteochondral tissue
Cell‐laden biphasic scaffolds with anisotropic structure for the regeneration of osteochondral tissue
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用于骨软骨组织再生的具有各向异性结构的充满细胞的双相支架
DOI：
10.1002/term.1879
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发表时间：
2014
影响因子：
3.3
通讯作者：
Gelinsky
中科院分区：
文献类型：
--
作者：
Schütz;Despang;Gelinsky
充分治疗软骨和骨软骨缺损以恢复相应组织的功能在再生医学中仍然具有挑战性。模拟骨和软骨特性的双相支架适合于同时再生两种组织。本研究描述了基于藻酸盐的双相但整体式支架的开发，其适合于在软骨部分中包埋活细胞。支架是在无菌和细胞相容性条件下根据扩散控制的定向离子凝胶化原理制造的，其导致形成通道样平行排列的孔，贯穿双相构建体的整个长度。合成过程导致各向异性结构，因为它在许多天然组织中发现。支架的两个不同层的特征在于不同的微观结构和机械性能，其为细胞形成相应的组织提供了合适的环境。人软骨细胞和人间充质干细胞在水凝胶形成过程中嵌入双相支架的软骨层内，并成功诱导其软骨形成（再）分化。尽管未诱导的人间充质干细胞的活力在培养期间下降，但在3周的整个培养期间，人软骨细胞和软骨诱导的人间充质干细胞的细胞活力在支架内保持较高，证明成功制造了载有细胞的厘米级结构，可用于骨软骨缺损的再生治疗。版权所有© 2014约翰威利父子有限公司.
Sufficient treatment of chondral and osteochondral defects to restore function of the respective tissue remains challenging in regenerative medicine. Biphasic scaffolds that mimic properties of bone and cartilage are appropriate to regenerate both tissues at the same time. The present study describes the development of biphasic, but monolithic scaffolds based on alginate, which are suitable for embedding of living cells in the chondral part. Scaffolds are fabricated under sterile and cell‐compatible conditions according to the principle of diffusion‐controlled, directed ionotropic gelation, which leads to the formation of channel‐like, parallel aligned pores, running through the whole length of the biphasic constructs. The synthesis process leads to an anisotropic structure, as it is found in many natural tissues. The two different layers of the scaffolds are characterized by different microstructure and mechanical properties which provide a suitable environment for cells to form the respective tissue. Human chondrocytes and human mesenchymal stem cells were embedded within the chondral layer of the biphasic scaffolds during hydrogel formation and their chondrogenic (re)differentiation was successfully induced. Whereas viability of non‐induced human mesenchymal stem cells decreased during culture, cell viability of human chondrocytes and chondrogenically induced human mesenchymal stem cells remained high within the scaffolds over the whole culture period of 3 weeks, demonstrating successful fabrication of cell‐laden centimetre‐scaled constructs for potential application in regenerative treatment of osteochondral defects. Copyright © 2014 John Wiley & Sons, Ltd.