Characterization of Circular RNAs in Chinese Buffalo (Bubalus bubalis) Adipose Tissue: A Focus on Circular RNAs Involved in Fat Deposition
Characterization of Circular RNAs in Chinese Buffalo (Bubalus bubalis) Adipose Tissue: A Focus on Circular RNAs Involved in Fat Deposition
复制标题
中国水牛 (Bubalus bubalis) 脂肪组织中环状 RNA 的表征：关注参与脂肪沉积的环状 RNA
DOI：
10.3390/ani9070403
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发表时间：
2019-07-01
期刊：
影响因子：
3
通讯作者：
Ma, Yun
中科院分区：
文献类型：
--
作者：
Huang, Jieping;Zhao, Jinhui;Ma, Yun
布法罗在东南亚和中东的几个国家和非洲发挥着至关重要的作用。脂肪沉积由于其对肉质的重要性而受到越来越多的关注。然而，关于布法罗脂肪组织发育的信息知之甚少，需要进一步调查。本研究对布法罗不同发育阶段脂肪组织的环状RNA（circRNA）进行了分析。共表达分析和功能富集揭示了相当数量的可能在脂肪沉积中起作用的circRNA。进一步的qRT-PCR分析鉴定了与PR/SET结构域16（脂肪代谢基因）显著相关的两个circRNA。我们的研究结果确定了布法罗脂肪沉积的候选调节因子，这可能对布法罗育种有价值。摘要环状RNA（circRNA）是一类参与多种生物过程的新型调控因子。然而，对布法罗中具有潜在脂肪沉积功能的circRNA知之甚少。本研究利用Illumina HiSeq 3000平台构建了3头青年水牛和3头成年水牛脂肪组织的6个RNA文库，并进行了双末端RNA测序。计算鉴定了总共5141个circRNA。其中，252个circRNA差异表达（DE）之间的青年和成年水牛。其中，54个在成人组中上调，198个下调。通过定量实时PCR（qRT-PCR）和桑格测序进一步鉴定了8种DE circRNA。共表达分析显示，34种circRNA与脂肪沉积相关基因表现出强相关性（|R|> 0.980）。其中，两种circRNA（19：45387150）之间的表达相关性|45389986和21：6969877| 69753491）和PR/SET结构域16进一步使用qRT-PCR验证，并且揭示了强相关性（1 >| R|> 0.8）。这些结果强烈表明，circRNA 19：45387150| 45389986和21：6969877| 69753491是布法罗脂肪沉积的潜在调节剂。综上所述，本研究首次对不同发育阶段脂肪组织的circRNA谱进行了表征，并揭示了两个与脂肪沉积相关基因强相关的circRNA，为布法罗脂肪沉积提供了新的候选调控因子。
Simple Summary Buffalo play a vital role in several southeastern and middle-eastern Asian countries and Africa. Fat deposition has received increased attention because of its importance to meat quality. However, information on the development of buffalo adipose tissue is poorly understood and requires further investigation. This study characterized circular RNA (circRNA) profiles of adipose tissues in different developmental stages in buffalo. Co-expression analysis and functional enrichment revealed a considerable number of circRNAs which may function in fat deposition. Further qRT-PCR analysis identified two circRNAs with a significant association with PR/SET domain 16, a fat metabolism gene. Our results identified candidate regulators of fat deposition in buffalo, which may be valuable for buffalo breeding. Abstract Circular RNAs (circRNAs) have been identified as a novel type of regulators involved in multiple biological processes. However, circRNAs with a potential function in fat deposition in buffalo are poorly understood. In this study, six RNA libraries of adipose tissue were constructed for three young and three adult Chinese buffaloes with paired-ends RNA sequencing using the Illumina HiSeq 3000 platform. A total of 5141 circRNAs were computationally identified. Among them, 252 circRNAs were differentially expressed (DE) between the young and adult buffaloes. Of these, 54 were upregulated and 198 were downregulated in the adult group. Eight DE circRNAs were further identified by quantitative real-time-PCR (qRT-PCR) and Sanger sequencing. Co-expression analysis revealed that 34 circRNAs demonstrated a strong correlation with fat deposition-associated genes (|r| > 0.980). Among these, expressional correlation between two circRNAs (19:45387150|45389986 and 21:6969877|69753491) and PR/SET domain 16 was further verified using qRT-PCR, and a strong correlation was revealed (1 > |r| > 0.8). These results strongly suggest that circRNAs 19:45387150|45389986 and 21:6969877|69753491 are potential regulators of buffalo fat deposition. In summary, this study characterized the circRNA profiles of adipose tissues at different stages for the first time and revealed two circRNAs strongly correlated with fat deposition-associated genes, which provided new candidate regulators for fat deposition in buffalo.