Costus Pictus Extracts Stimulate Insulin Secretion from Mouse and Human Islets of Langerhans In Vitro
Costus Pictus Extracts Stimulate Insulin Secretion from Mouse and Human Islets of Langerhans In Vitro
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DOI：
10.1159/000324007
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发表时间：
2010-01-01
影响因子：
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通讯作者：
Persaud, Shanta J.
中科院分区：
文献类型：
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作者：
Al-Romaiyan, Altaf;Jayasri, Mangalam A.;Persaud, Shanta J.
几个世纪以来，植物提取物一直被用作治疗 2 型糖尿病 (T2DM) 的民间疗法，并为发展中国家的传统药物提供了廉价且易于获得的替代品。据报道，木香 (CP) 是一种属于木香科的植物，其提取物具有体内抗糖尿病活性。 CP 的确切分子作用模式尚不清楚，但动物研究中发现的降血糖作用与胰岛素分泌的急剧增加有关，因此在我们的研究中，我们测量了 CP 水提取物对 MIN6 细胞系以及分离的小鼠和人类胰岛的体外胰岛素分泌的影响。我们的数据表明，CP 对基础胰岛素分泌有直接刺激作用，但对刺激葡萄糖浓度没有影响，这与膜完整性受损或细胞活力降低无关。单细胞钙微荧光测定表明，CP 引起细胞内 Ca(2+) 浓度 ([Ca(2+)](i)) 升高，这种效应可通过去除细胞外 Ca(2+) 或阻断电压门控 Ca(2+) 通道 (VGCC) 完全消除。这些体外观察表明，CP 的一种作用模式是通过刺激胰岛素分泌，这可能部分是由 CP 通过 VGCC 增加 [Ca(2+)](i) 水平的能力介导的。 CP 提取物可能为治疗 T2DM 患者提供一种负担得起且廉价的替代方案。版权所有 (C) 2010 S. Karger AG，巴塞尔
Plant-derived extracts have been used as folk remedies for Type 2 diabetes mellitus (T2DM) for many centuries, and offer the potential of cheap and readily available alternatives to conventional pharmaceuticals in developing countries. Extracts of Costus pictus (CP), a plant belonging to the Costaceae family, are reported to have antidiabetic activity in vivo. The exact molecular mode of action(s) of CP is unclear but the antihyperglycemic effect seen in animal studies was associated with dramatic increases in insulin secretion so in our study we have measured the effect of aqueous CP extract on insulin secretion in vitro from the MIN6-cell line and isolated mouse and human islets. Our data demonstrate that CP has a direct stimulatory effect on insulin secretion at basal but not stimulatory glucose concentrations which was not associated with compromised membrane integrity or decrease-cell viability. Single cell calcium microfluorimetry measurements showed that CP caused elevations in-cell intracellular Ca(2+) concentrations ([Ca(2+)](i)), an effect which was completely abolished by the removal of extracellular Ca(2+) or blockade of voltage-gated Ca(2+) channels (VGCC). These in vitro observations suggest that one mode of action of CP is through stimulating insulin secretion which may be mediated, in part, by the ability of CP to increase [Ca(2+)](i) levels through VGCC. CP extracts may provide an affordable and inexpensive alternative for treating patients with T2DM. Copyright (C) 2010 S. Karger AG, Basel