Simple, recurring RNA binding sites for L-arginine
Simple, recurring RNA binding sites for L-arginine
复制标题
DOI：
10.1261/rna.1979410
复制
发表时间：
2010-04-01
期刊：
影响因子：
4.5
通讯作者：
Yarus, Michael
中科院分区：
文献类型：
--
作者：
Janas, Teresa;Widmann, Jeremy Joseph;Yarus, Michael
从包含 17、25 和 50mer 随机片段的异质 RNA 池中选出了 7 个新的精氨酸结合基序，产生了 131 个独立衍生的多重分离的结合位点。最短的 17mer 随机区域足以使用五个不同的保守基序（基序 1a、1b、1c、2 和 4）构建不同的精氨酸结合位点。解离常数在分数毫摩尔至毫摩尔范围内。结合位点是氨基酸侧链特异性的，并且适度区分精氨酸的 L- 和 D- 立体异构体，表明分子集中在侧链胍上。精氨酸编码三联体（密码子/反密码子）在最大的 Arg 位点家族（占所有序列的 72%）中高度保守，在 Ile、His 和 Trp 的最小、最常见的 RNA 结合位点中也发现了这一点。
Seven new arginine binding motifs have been selected from a heterogeneous RNA pool containing 17, 25, and 50mer randomized tracts, yielding 131 independently derived binding sites that are multiply isolated. The shortest 17mer random region is sufficient to build varied arginine binding sites using five different conserved motifs (motifs 1a, 1b, 1c, 2, and 4). Dissociation constants are in the fractional millimolar to millimolar range. Binding sites are amino acid side-chain specific and discriminate moderately between L- and D-stereoisomers of arginine, suggesting a molecular focus on side-chain guanidinium. An arginine coding triplet (codon/anticodon) is highly conserved within the largest family of Arg sites (72% of all sequences), as has also been found in minimal, most prevalent RNA binding sites for Ile, His, and Trp.