Introduce of Quick,Easy,Cheap,Effective,Rugged and Safe(QuEchERS) Approach for Determining Pesticide Residues
Introduce of Quick,Easy,Cheap,Effective,Rugged and Safe(QuEchERS) Approach for Determining Pesticide Residues
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发表时间：
2006
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通讯作者：
Ding-jin Hu
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作者：
Ding-jin Hu
本章介绍了一种简单、快速、廉价的方法，用于测定食品和其他潜在基质中的农药。该方法被称为快速、简单、廉价、有效、坚固和安全（QuEchERS）的农药残留检测方法，包括用含有1%乙酸（Hac）的乙腈（MeCN）提取样品，并通过加入无水硫酸镁（MgSO 4）和乙酸钠（NaAc）形成液-液分配，然后进行简单的净化步骤，称为分散固相萃取（SPE）。QuEchERS方法通过振摇Teflon离心管进行，该离心管含有1 mL的1%Hac的MeCN溶液加上0.4g无水乙醇。MgSO 4和0.1 g无水NaAc/样品。然后离心该管，并将一部分提取物转移到含有50 mg伯仲胺（PSA）吸附剂加150 mg无水乙醇的管中。MgSO 4/mL浸提液（分散SPE净化步骤）。然后将提取物转移到自动进样器小瓶中，进行GC/MS和LC/MS-MS的同时分析。通过可选仪器、LOQ、分析范围和不同基质调整替代品。
This chapter describes a simple, fast, and inexpensive method for the determination of pesticides in foods and potentially other matrices. The method, known as the quick, easy, cheap, effective, rugged, and safe (QuEchERS) method for pesticide residues involves the extraction of the sample with acetonitrile (MeCN) containing 1% acetic acid (Hac) and simultaneous liquid-liquid partitioning formed by adding anhydrous magnesium sulfate (MgSO4) plus sodium acetate (NaAc) following by a simple cleanup step known as dipersive solid-phase extraction (SPE). The QuEchERS method is carried out by shaking a Teflon centrifuge tube which contains 1mL of 1% Hac in MeCN plus 0.4g anh. MgSO4 and 0.1 g anh NaAc per sample. The tube is then centrifuged, and a partion of the extract is transferred to a tube containing 50 mg primary secondary amine (PSA) sorbent plus 150 mg anh. MgSO4 per mL extract (the dispersive-SPE cleanup step). Then, the extract is transferred to autosampler vials for concurrent analysis by GC/MS and LC/MS-MS. Estimation of the method’ stability and reliability and its use was done too. Alternatives was adjusted by optional instruments, LOQ, analytical scope and different matrices.