In vitro reconstructed human epithelia reveal contributions of Candida albicans EFG1 and CPH1 to adhesion and invasion
In vitro reconstructed human epithelia reveal contributions of Candida albicans EFG1 and CPH1 to adhesion and invasion
复制标题
DOI：
10.1099/00221287-148-2-497
复制
发表时间：
2002-02-01
期刊：
影响因子：
2.8
通讯作者：
Rupp, S
中科院分区：
文献类型：
--
作者：
Dieterich, C;Schandar, M;Rupp, S
两种转录因子 EFG1 和 CPH1 的单独和协同贡献已在白色念珠菌对人类上皮细胞的粘附和侵袭方面得到表征。为此目的，开发了两种体外重建组织模型。人体表皮的多层模型用于模拟皮肤的浅表感染，而重建的人体肠道模型用于模拟全身感染的第一步。结果表明，与同源临床分离株 Sc5314 相比，删除转录因子 CPH1 和 EFG1 的白色念珠菌既不能粘附也不能穿透任一模型系统。单独删除 EFG1 的菌株显示粘附力显着降低，并且无法穿透角质层。然而，删除 CPH1 的菌株显示出与临床分离株 Sc5314 相似的表型。使用体外重建的不同类型的多层人体组织，可以定义 Efg1p 和 Cph1p 对白色念珠菌两个重要毒力因子（即粘附和侵袭）的单独贡献。
The individual and synergistic contributions of two transcription factors, EFG1 and CPH1, have been characterized with regard to adhesion to, and invasion of, human epithelia by Candida albicans. For this purpose two in vitro reconstructed tissue models were developed. A multi-layered model of human epidermis was used to simulate superficial infections of the skin, whereas a reconstructed human intestinal model was used to mimic the first steps of systemic infections. It was shown that C. albicans deleted for both transcription factors CPH1 and EFG1, in contrast to the congenic clinical isolate Sc5314, was neither able to adhere to, nor to penetrate, either of the model systems. A strain deleted for EFG1 alone showed significant reduction in adhesion and was not able to penetrate through the stratum corneum. However, strains deleted for CPH1 showed phenotypes paralleling the phenotypes of the clinical isolate Sc5314. Using different types of multilayered human tissues reconstructed in vitro the individual contributions of Efg1p and Cph1p to two important virulence factors of C. albicans, namely adhesion and invasion, could be defined.