Qualitative and quantitative comparison of cyclic phosphatidic acid and its related lipid species in rat serum using hydrophilic interaction liquid chromatography with tandem-mass spectrometry
Qualitative and quantitative comparison of cyclic phosphatidic acid and its related lipid species in rat serum using hydrophilic interaction liquid chromatography with tandem-mass spectrometry
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DOI：
10.1016/j.chroma.2018.07.010
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发表时间：
2018-09-14
影响因子：
4.1
通讯作者：
Murakami-Murofushi, Kimiko
中科院分区：
文献类型：
--
作者：
Fukasawa, Keiko;Nakajima, Shingo;Murakami-Murofushi, Kimiko
环状磷脂酸（cPA）是一种简单的脂质，含有连接在sn-1位的脂肪酸和甘油骨架sn-2和sn-3位的环状磷酸酯环结构。cPA的药理学作用已在几种疾病如癌症和神经性疼痛中得到证实;然而，相对于生物样品中的其他脂质种类，cPA的分子种类的组成仍不清楚。最近，亲水相互作用液相色谱法（HILIC）已经证明了进行生物样品的脂质组学分析的能力。在本研究中，我们开发了定量测定cPA及其相关的脂质物种，如溶血磷脂酸（LPA）和溶血磷脂酰胆碱（LPC），在大鼠血清中使用HILIC配备串联质谱（MS/MS）。采用HILIC-MS/MS系统进行脂质分析，线性好，重复性好。结果表明，柠檬酸改良Bligh和Dyer法提取cPA和LPA的效率较高，且不污染人工产物。在大鼠血清中，cPA和LPC含有更多的饱和脂肪酸，如棕榈酸和硬脂酸比不饱和脂肪酸，而LPA和磷脂酰胆碱含有更多的不饱和脂肪酸比饱和脂肪酸。本研究中测定cPA及其相关脂质物质的分析方法将有助于分析其代谢。(C)2018 Elsevier B. V.版权所有。
Cyclic phosphatidic acid (cPA) is a simple lipid containing a fatty acid attached at the sn-1 position and a cyclic phosphate ring structure at the sn-2 and sn-3 positions of the glycerol backbone. The pharmacological effects of cPA have been demonstrated in several diseases such as cancer and neuropathic pain; however, the composition of the molecular species of cPA in relative to other lipid species in biological samples is still unclear. Recently, hydrophilic interaction liquid chromatography (HILIC) has demonstrated the ability to perform lipidomic analyses of biological samples. In the present study, we developed the quantitative measurement of cPA and its related lipid species, such as lysophosphatidic acid (LPA) and lysophosphatidylcholine (LPC), in rat serum using HILIC equipped with tandem-mass spectrometry (MS/MS). The lipid analysis using HILIC-MS/MS system demonstrated high linearity and reproducibility. The modified Bligh and Dyer method using citric acid was showed high efficiency on the extraction of cPA and LPA without contamination of artificial products. In rat serum, cPA and LPC contained more saturated fatty acids such as palmitic acid and stearic acid than unsaturated fatty acids, whereas LPA and phosphatidylcholine more contained unsaturated fatty acids than saturated fatty acids. The analytical methods for measuring cPA and its related lipid species in the present study will aid the analysis of their metabolism. (C) 2018 Elsevier B.V. All rights reserved.