Early infection of scutellum tissue with Agrobacterium allows high-speed transformation of rice
Early infection of scutellum tissue with Agrobacterium allows high-speed transformation of rice
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DOI：
10.1111/j.1365-313x.2006.02836.x
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发表时间：
2006-09-01
期刊：
影响因子：
7.2
通讯作者：
Tanaka, Hiroshi
中科院分区：
文献类型：
--
作者：
Toki, Seiichi;Hara, Naho;Tanaka, Hiroshi
最近采用了几种方法来改善农业介导的水稻的转化，两者都产生了大量的稻米基因组功能分析所需的T-DNA插入植物，以及生产具有额外农艺价值的水稻。但是，分离转基因水稻植物仍然需要大约3个月的体外培养。在这里，我们报告了1天预培养的种子对农杆菌介导的转化的能力。此外，用农杆菌早期感染了水稻种子，可提高有效的转化Calli。使用我们的系统，我们在成熟种子无菌培养的一个月内成功再生转基因水稻植物。我们的新系统应减少伴随着去分化状态的水稻细胞延长培养的somaclonal变异，并促进水稻的分子育种。
Several approaches have recently been adopted to improve Agrobacterium-mediated transformation of rice, both to generate the large number of T-DNA insertion plants needed for functional analysis of the rice genome, and for production of rice with additional agronomical value. However, about 3 months of in vitro culture is still required for isolation of transgenic rice plants. Here, we report the competency of scutellum tissue from 1-day pre-cultured seeds for Agrobacterium-mediated transformation. Furthermore, early infection of rice seeds with Agrobacterium enhanced efficient selection of transformed calli. Using our system, we successfully regenerated transgenic rice plantlets within a month of the start of the aseptic culture of mature seeds. Our new system should reduce the somaclonal variation accompanying prolonged culture of rice cells in the dedifferentiated state and facilitate the molecular breeding of rice.