Hypouricemic effect of the methanol extract from Prunus mume fruit in mice
Hypouricemic effect of the methanol extract from Prunus mume fruit in mice
复制标题
梅果甲醇提取物对小鼠的降尿酸作用
DOI：
10.3109/13880209.2012.683115
复制
发表时间：
2012
影响因子：
3.8
通讯作者：
Cheng
中科院分区：
文献类型：
--
作者：
Li;Jing Li;Dong;Jingfang Dong;Cheng
上下文：李子的果实。枸杞（蔷薇科）在东亚国家作为保健食品或传统中草药中具有悠久的药用历史。目的：研究梅果甲醇提取物（MPMF）对氧酸钾致高尿毒症小鼠的降尿酸作用。材料与方法：观察MPMF（35、70、140 mg/kg, p.o）给药7 d对小鼠血清、肝脏、尿尿酸水平及肝脏黄嘌呤氧化酶（XO）活性的影响。结果：氧酸钾诱导的高尿酸血症小鼠血清和肝脏尿酸水平升高（11.0 mg/dL和0.52 mg/g组织），尿尿酸水平降低（49.9 mg/dL）。口服140 mg/kg MPMF 7天可逆转血清、肝脏和尿尿酸水平的异常（分别为7.1 mg/dL、0.37 mg/g组织和69.7 mg/dL）。此外，与高尿酸血症小鼠（3.9 nmol/min / mg蛋白）相比，70和140 mg/kg MPMF（3.1和2.9 nmol/min / mg蛋白）可抑制肝脏XO活性。讨论与结论：结果表明，MPMF的有益降尿酸作用可能是通过抑制肝脏XO活性介导的，至少部分是通过抑制肝脏XO活性介导的。我们的研究表明，梅子及其提取物可能具有相当大的发展潜力，作为抗痛风药物的临床应用。
Context: The fruit of the Prunus mume Sieb. et Zucc (Rosaceae) is used as a health food or medicinal material in traditional herb medicine for a long time in Eastern Asian countries. Objective: Our present study investigated the hypouricemic effect of the methanol extract from P. mume fruit (MPMF) in mice with potassium oxonate-induced hyperuremia. Materials and methods: Effect of MPMF (35, 70 and 140 mg/kg, p.o.) administrated for 7 days on the serum, liver, urinary uric acid levels and liver xanthine oxidase (XO) activity were assessed in mice. Results: Hyperuricemic mice induced by potassium oxonate demonstrated an elevation in serum and liver uric acid levels (11.0 mg/dL and 0.52 mg/g tissue) and a reduction in urinary uric acid levels (49.9 mg/dL). Oral administration of 140 mg/kg MPMF for 7 days reversed the abnormalities in serum, liver and urinary uric acid levels (7.1 mg/dL, 0.37 mg/g tissue and 69.7 mg/dL, respectively). In addition, 70 and 140 mg/kg MPMF (3.1 and 2.9 nmol/min per mg protein) inhibited liver XO activity compared with hyperuricemic mice (3.9 nmol/min per mg protein). Discussion and conclusion: The results indicated that the beneficial hypouricaemic effect of MPMF may be mediated, at least in part, by inhibiting XO activity in the liver. Our study suggests that P. mume and its extracts may have a considerable potential for development as an anti-gout agent for clinical application.