Production of recombinant Von Willebrand factor by CHO cells cultured in macroporous microcarriers
Production of recombinant Von Willebrand factor by CHO cells cultured in macroporous microcarriers
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利用大孔微载体培养的 CHO 细胞生产重组血管性血友病因子
DOI：
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发表时间：
1990
期刊：
影响因子：
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通讯作者：
J. Romet‐Lemonne
中科院分区：
文献类型：
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作者：
G. Mignot;T. Faure;V. Ganne;B. Arbeille;A. Pavirani;J. Romet‐Lemonne
在明胶大孔微载体（Cultispher-G）中成功培养了产生血管性血血病因子的重组中国仓鼠卵巢细胞。无血清培养物在1、4和10升发酵罐中保持两个月以上。在1升发酵罐中进行了与Cytodex-3微载体的比较研究。在Cultispher-G上培养的CHO细胞特异性血管性血液病因子的较低生产率被较高的细胞密度（107−2×107细胞/ml）所抵消。体积冯氏血友病因子产率受氧浓度的影响，在1 ~ 10升的发酵过程中保持稳定。
Recombinant Chinese hamster ovary cells producing Von Willebrand factor have been successfully grown in gelatin macroporous microcarriers (Cultispher-G). Serum-free cultures were maintained in 1, 4, and 10 liter fermentors for more than two months. Comparative studies with Cytodex-3 microcarriers have been performed in 1 liter fermentors. The lower specific Von Willebrand factor productivity of CHO cells cultivated on Cultispher-G were offset by higher cell densities (107−2×107 cells/ml). Volumetric Von Willebrand factor productivity was influenced by oxygen concentration, and remained stable during scale-up from 1 to 10 liter fermentors.