Identification of Genes Uniquely Expressed in the Germ-Line Tissues of the Jewel Wasp Nasonia vitripennis.
Identification of Genes Uniquely Expressed in the Germ-Line Tissues of the Jewel Wasp Nasonia vitripennis.
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DOI：
10.1534/g3.115.021386
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发表时间：
2015-10-13
期刊：
影响因子：
--
通讯作者：
Akbari OS
中科院分区：
文献类型：
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作者：
Ferree PM;Fang C;Mastrodimos M;Hay BA;Amrhein H;Akbari OS
珠宝蜂（Nasonia vitripennis）是研究膜翅目昆虫单倍体-二倍体繁殖特征的新兴模式生物，包括所有的黄蜂、蜜蜂和蚂蚁。我们对玻璃蜂的卵巢、雌性体细胞和雄性体细胞进行了转录谱分析，以补充之前存在的黄蜂睾丸转录组。这些数据被储存到一个开放获取的基因组浏览器中，用于可视化转录本相对于它们的基因模型。我们使用这些数据来鉴定在种系组织中独特表达的基因组合。我们发现156个蛋白编码基因在黄蜂的睾丸中完全表达，而在卵巢中只有22个。在睾丸特异性基因中，有8个是男性特异性DNA包装蛋白——精蛋白的候选基因。我们在睾丸和卵巢中发现了非常相似的中心体相关基因的表达模式，这表明新生中心体的形成（未受精卵发育为雄性的关键过程）可能不依赖于这些组织之间的大规模转录差异。相反，许多减数分裂相关的基因显示出偏向于睾丸特异性表达，尽管在玻璃羽衣库雄性中缺乏真正的减数分裂。这些模式可能反映了这种生物的单倍体雄性雄性配子产生的意想不到的复杂性。总的来说，这些数据增加了越来越多的基因组和遗传工具，用于解决这一新兴昆虫模式生物的重要生物学问题。
The jewel wasp Nasonia vitripennis is a rising model organism for the study of haplo-diploid reproduction characteristic of hymenopteran insects, which include all wasps, bees, and ants. We performed transcriptional profiling of the ovary, the female soma, and the male soma of N. vitripennis to complement a previously existing transcriptome of the wasp testis. These data were deposited into an open-access genome browser for visualization of transcripts relative to their gene models. We used these data to identify the assemblies of genes uniquely expressed in the germ-line tissues. We found that 156 protein-coding genes are expressed exclusively in the wasp testis compared with only 22 in the ovary. Of the testis-specific genes, eight are candidates for male-specific DNA packaging proteins known as protamines. We found very similar expression patterns of centrosome associated genes in the testis and ovary, arguing that de novo centrosome formation, a key process for development of unfertilized eggs into males, likely does not rely on large-scale transcriptional differences between these tissues. In contrast, a number of meiosis-related genes show a bias toward testis-specific expression, despite the lack of true meiosis in N. vitripennis males. These patterns may reflect an unexpected complexity of male gamete production in the haploid males of this organism. Broadly, these data add to the growing number of genomic and genetic tools available in N. vitripennis for addressing important biological questions in this rising insect model organism.