Seroprevalence of porcine epidemic diarrhea virus infection among different types of breeding swine farms in Spain
Seroprevalence of porcine epidemic diarrhea virus infection among different types of breeding swine farms in Spain
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DOI：
10.1016/0167-5877(94)00429-m
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发表时间：
1995-05
影响因子：
2.6
通讯作者：
Cármenes P
中科院分区：
文献类型：
--
作者：
Carvajal A;Lanza I;Diego R;Rubio P;Cármenes P
对西班牙卡斯蒂利亚-莱昂地区养猪场中猪流行性腹泻病毒(PEDV)感染的血清流行率进行了横断面研究。养殖场根据规模和生产类型(集约型或粗放型)进行分层。根据现有的最新普查数据和其他国家获得的血清阳性农场的流行率，计算出每一层要抽样的农场数量。根据我们在以前的研究中获得的猪群内血清阳性率数据，在每个猪群中，对检测疾病存在所需的母猪数量进行了抽样。1992-1993年收集了来自794个不同养殖场的5098份母猪血清样本，用单抗阻断酶联免疫吸附试验检测了PEDV特异性抗体的存在；55.9%的猪群至少有一只动物呈阳性；29.6%的血清呈阳性。在整个采样地区都检测到了血清阳性的养殖场，这表明感染很普遍。与小型猪场(≤，20头)相比，20头以上母猪的农场患病率更高，集约化猪群的猪场感染率更高。另一方面，随着农场规模的扩大，农场内的血清阳性率和阳性血清的平均阻断百分率下降。
A cross-sectional study for determining the seroprevalence of porcine epidemic diarrhea virus (PEDV) infection among swine breeding farms in the Castilla y León region (Spain) was carried out. Breeding farms were stratified according to size and type of production (intensive or extensive). The number of farms to be sampled in each stratum was calculated from the latest census data available and the prevalence of seropositive farms obtained in other countries. Within each herd, the number of sows needed to detect the presence of the disease were sampled, according to within-herd seroprevalence data obtained by us in previous studies. A total of 5098 sow serum samples from 794 different farms were collected during 1992–1993 and tested for the presence of PEDV-specific antibodies, using a blocking ELISA with monoclonal antibodies; 55.9% of herds had at least one positive animal; 29.6% of sera were positive. Seropositive farms were detected throughout the sampled region, indicating that the infection was widespread. Higher farm-level prevalence rates were found in farms with more than 20 sows compared with small ones (≤ 20 sows) and in intensive than in extensive herds. On the other hand, within-farm seroprevalence and mean blocking percentage of positive sera decreased with increasing size of the farm.