Perfluorooctanoic acid-induced liver injury is potentially associated with gut microbiota dysbiosis
Perfluorooctanoic acid-induced liver injury is potentially associated with gut microbiota dysbiosis
复制标题
全氟辛酸引起的肝损伤可能与肠道微生物群失调有关。
DOI：
10.1016/j.chemosphere.2020.129004
复制
发表时间：
2021-01-06
期刊：
影响因子：
8.8
通讯作者：
Chen, Wei
中科院分区：
文献类型：
--
作者：
Wang, Gang;Pan, Ruili;Chen, Wei
全氟辛酸（PFOA）是一种环境污染物，广泛存在于工业产品中，容易在肝脏中积累。新出现的证据表明，肠道微生物组是维持动物健康的关键因素，可能会被外源性物质改变。然而，很少有研究探讨PFOA诱导的肝损伤是否与肠道微生物群失调有关。在本研究中，研究了亚急性和亚慢性PFOA暴露对C57 BL/6 J小鼠肝脏和肠道微生物群的影响。我们的研究结果表明，亚急性和亚慢性暴露于PFOA诱导肝脏炎症，破坏抗氧化稳态，并导致肝脏组织学异常，可检测到肝肿大，最终引发肝损伤。此外，16 S rRNA测序分析显示，亚急性PFOA暴露导致已知导致肝脏炎症和氧化应激的肠道植物群的丰度发生显著变化，例如脱盐杆菌属和拟杆菌属。与未处理组相比，暴露于亚慢性毒性主要导致包括乳杆菌属和双歧杆菌属在内的肠道益生菌的减少，这些益生菌可能对肝损伤有益。它们还导致了通过重建未观察状态的群落系统发育调查（PICRUSt）分析的微生物群落的功能能力受到干扰。此外，短链脂肪酸（SCFAs）的水平，特别是丁酸，通过PFOA管理显着降低。总的来说，我们的观察结果表明，亚急性和亚慢性PFOA暴露引起的肝损伤可能部分与肠道微生物群失调有关，并为PFOA在肝损伤中的作用提供了新的见解。(C)2020爱思唯尔有限公司保留所有权利。
Perfluorooctanoic acid (PFOA), an environmental pollutant, is widely engaged in industrial products and tends to accumulate in the liver. Emerging evidence has suggested that the gut microbiome is a pivotal player in maintaining animal health and can potentially altered by xenobiotic. However, few studies explored whether PFOA-induced liver injury is associated with gut microbiota dysbiosis. In the present study, the effects of subacute and subchronic PFOA exposure on liver and gut microbiota in C57BL/6J mice were investigated. Our findings showed that both subacute and subchronic exposure to PFOA induced the liver inflammation, disrupted antioxidative homeostasis and caused liver histological abnormalities with detectable hepatomegaly, ultimately triggering liver injury. Besides, 16S rRNA sequencing analysis revealed that subacute PFOA exposure caused significant changes in the abundances of intestinal flora known to contribute to liver inflammation and oxidative stress, such as the Dehalobacterium and Bacteroides genera. Exposure to subchronic toxicity mainly induced the decrease in commensal probiotics including Lactobacillus and Bifidobacterium genera, which are potentially beneficial to liver damage, compared with that in the untreated group. They also resulted in disturbed functional capabilities of the microbial communities by a Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) analysis. Additionally, the levels of short-chain fatty acids (SCFAs), especially butyric acid, were significantly reduced by PFOA administration. Collectively, our observations suggested that liver damage induced by both subacute and subchronic PFOA exposures probably partly related to the gut microbiota dysbiosis and provided a new insight into the role of PFOA in liver injury. (C) 2020 Elsevier Ltd. All rights reserved.