Differentiation-Associated MicroRNA Alterations in Mouse Heart-Derived Sca-1(+)CD31(-) and Sca-1(+)CD31(+) Cells.
Differentiation-Associated MicroRNA Alterations in Mouse Heart-Derived Sca-1(+)CD31(-) and Sca-1(+)CD31(+) Cells.
复制标题
小鼠心脏来源的 Sca-1( )CD31(-) 和 Sca-1( )CD31( ) 细胞中分化相关的 MicroRNA 改变。
DOI：
10.1155/2016/9586751
复制
发表时间：
2016
影响因子：
4.3
通讯作者：
Zhou Z
中科院分区：
文献类型：
--
作者：
Wu Q;Zhan J;Li Y;Wang X;Xu L;Yu J;Pu S;Zhou Z
心脏驻留干/祖细胞（CSC/CPCs）对心脏的细胞和功能完整性至关重要，因为它们维持心肌细胞的稳态。基于不同干细胞相关抗原的表达，已经鉴定了几个CSC/CPC群体。心肌组织中Sca-1+细胞可能是最常见的CSC/CPC。然而，它们是异质细胞群，在移植中，临床医生可能会移植更多的内皮细胞，心肌细胞或其他细胞而不是干细胞。本研究的目的是：（1）使用流式细胞分选法分离具有Lin− CD 45 −Sca-1+ CD 31 −和Lin− CD 45 −Sca-1+ CD 31+表面抗原的CSC/CPC;（2）研究其分化潜力;（3）确定细胞亚型之间干细胞特征差异的分子基础。结果表明，小鼠心脏来源的Sca-1+ CD 31 −细胞是多能的，并保留了分化为不同心脏细胞谱系的能力，但Sca-1+ CD 31+细胞没有。microRNA和mRNA表达的综合分析表明，20种microRNA和49种mRNA与Sca-1+ CD 31 −和Sca-1+ CD 31+亚型干性特征呈负相关。特别是mmu-miR-322- 5 p具有更多的靶向和负相关的基因和转录因子，可能具有更高的CSC/CPC分化潜力。
Cardiac resident stem/progenitor cells (CSC/CPCs) are critical to the cellular and functional integrity of the heart because they maintain myocardial cell homeostasis. Several populations of CSC/CPCs have been identified based on expression of different stem cell-associated antigens. Sca-1+ cells in the cardiac tissue may be the most common CSC/CPCs. However, they are a heterogeneous cell population and, in transplants, clinicians might transplant more endothelial cells, cardiomyocytes, or other cells than stem cells. The purposes of this study were to (1) isolate CSC/CPCs with Lin−CD45−Sca-1+CD31− and Lin−CD45−Sca-1+CD31+ surface antigens using flow-activated cell sorting; (2) investigate their differentiation potential; and (3) determine the molecular basis for differences in stemness characteristics between cell subtypes. The results indicated that mouse heart-derived Sca-1+CD31− cells were multipotent and retained the ability to differentiate into different cardiac cell lineages, but Sca-1+CD31+ cells did not. Integrated analysis of microRNA and mRNA expression indicated that 20 microRNAs and 49 mRNAs were inversely associated with Sca-1+CD31− and Sca-1+CD31+ subtype stemness characteristics. In particular, mmu-miR-322-5p had more targeted and inversely associated genes and transcription factors and might have higher potential for CSC/CPCs differentiation.